BBa_J100209 1 BBa_J100209 tClone Red RS8 2015-06-01T11:00:00Z 2015-08-31T04:08:24Z The receiving plasmid is J119361 and the riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This sequence has Isolated pUC-IDT from tClone Red (J119361) by removing sequence in between the BsaI restriction sites and then RS8 riboswtich (from Wachsmuth et al.) was ligated. A ligand such as theophylline can bind to the sequence and allow transcription to take place. false false _578_ 0 23989 9 Not in stock false BsaI recognition site in the pUC-IDT. false Shuk Hang Li annotation2432580 1 P5 promoter range2432580 1 1 36 annotation2432583 1 RS8 riboswtich range2432583 1 41 112 annotation2432585 1 BD18 C dog RBS range2432585 1 115 201 annotation2432587 1 RFP range2432587 1 200 880 BBa_J100209_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacaagtgataccagcatcgtcttgatgcccttggcagcacttcactcctagtggagtgaagtgctgttttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z