BBa_J100210 1 BBa_J100210 tCloneRed with RS4 Transcriptional Riboswitch 2015-06-01T11:00:00Z 2015-08-31T04:08:24Z The destination vector is part J119361. The sequence of the riboswitch comes from the paper "De novo design of a synthetic riboswitch that regulates transcription termination," by Wachsmuth, et al. (referred to as "RS4" in that paper; doi:10.1093/nar/gks1330). Using Golden Gate Assembly, the transcriptional riboswitch RS4 (from Wachsmuth, et al.) was ligated into the destination vector tClone Red (J119361) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine. false false _578_ 0 18858 9 Not in stock false Appropriate sticky ends had to be added to the RS4 sequence in order to anneal with those created by digestion of the destination vector with BsaI. false Monica Prudencio annotation2432575 1 P5 Promoter range2432575 1 1 36 annotation2432576 1 RS4 Transcription Riboswitch range2432576 1 41 117 annotation2432578 1 BD18 C dog RBS range2432578 1 122 206 annotation2432581 1 RFP range2432581 1 207 887 BBa_J100210_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacaagtgataccagcatcgtcttgatgcccttggcagcacttcaaaccgaaatttgcgcttgaagtgctgcttttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z