BBa_J100211 1 BBa_J100211 tCloneTet+Red; Fusion Protein Reporter 2015-06-08T11:00:00Z 2015-08-31T04:08:24Z The sources are J119140 and J119361. The linker chain was developed from part K598018 "tetA+GFP fused protein" by Qingyang XIAO. This part was made from adding the TetA gene from part J119140 in front of the RFP gene in the tCloneRed part J119361. The Tet and RFP genes were combined, by deleting the "stop" sequence in the Tet gene and adding a linker sequence of proteins GGGS x4 to create a fusion protein. The resultant protein is a single protein with both the Tetracycline resistance protein and the Red fluorescent protein, combined with a linker chain. This part still contains tClone, and will not transcribe the reporter gene (Tet+Red) without the insertion of a riboswitch etc. between the BsaI sites. true false _578_ 0 23950 9 Discontinued false We did not know if the fusion protein would work with RFP as it was shown to do with GFP. false Julia Preziosi igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z