LOCUS       BBa_J100212             2133 bp    DNA     linear       23-Nov-2024
DEFINITION  tCloneTet+Red fusion protein reporter with Riboswitch 3
ACCESSION   BBa_J100212
VERSION     BBa_J100212.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 promoter
     stem_loop       41..121
                     /label=Riboswitch 3
     CDS             211..1404
                     /label=TetA
     CDS             1405..1452
                     /label=Linker sequence
     CDS             1453..2133
                     /label=RFP
     RBS             126..210
                     /label=BD18 C dog RBS
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac aagtgatacc agcatcgtct
       61 tgatgccctt ggcagcactt catttacata ctcggtaaac tgaagtgctg ccattttttt
      121 tgcgggggcc caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa
      181 acatcttaat catgcgacgg agcgtttcta atgggactta aatctaacaa tgcgctcatc
      241 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
      301 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
      361 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
      421 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
      481 atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc
      541 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      601 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      661 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      721 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      781 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      841 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      901 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
      961 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggc
     1021 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     1081 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     1141 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     1201 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     1261 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     1321 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1381 gcatggagcc gggccacctc gaccggcggt ggaagcggcg gcggctccgg tggtggttct
     1441 ggaggcggtt ctatggcttc ctccgaagat gttatcaaag agttcatgcg tttcaaagtt
     1501 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
     1561 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
     1621 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
     1681 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
     1741 atgaacttcg aagatggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
     1801 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
     1861 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agatggtgct
     1921 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
     1981 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
     2041 atcaaactgg acatcacctc ccacaacgaa gattacacca tcgttgaaca gtacgaacgt
     2101 gctgaaggtc gtcactccac cggtgcttaa taa
//