LOCUS       BBa_J100213             2152 bp    DNA     linear       23-Nov-2024
DEFINITION  tCloneTet+Red fusion protein reporter with Riboswitch 3Shift
ACCESSION   BBa_J100213
VERSION     BBa_J100213.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 promoter
     stem_loop       41..140
                     /label=Riboswitch 3 Shift
     RBS             145..229
                     /label=BD18 C dog RBS
     CDS             230..1423
                     /label=TetA
     CDS             1424..1471
                     /label=Linker sequence
     CDS             1472..2152
                     /label=RFP
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac aagtgatacc agcatcgtct
       61 tgatgccctt ggcagcactt catttacata ctcggtaaac tgaagtgctg ccattttttt
      121 taattcgagc tccgtcgaca gcgggggccc aagttcactt aaaaaggaga tcaacaatga
      181 aagcaatttt cgtactgaaa catcttaatc atgcgacgga gcgtttctaa tgggacttaa
      241 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
      301 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
      361 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
      421 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
      481 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
      541 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      601 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
      661 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
      721 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
      781 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
      841 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
      901 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
      961 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     1021 tcaagccttc gtcactggcc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     1081 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     1141 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     1201 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     1261 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc
     1321 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     1381 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg accggcggtg gaagcggcgg
     1441 cggctccggt ggtggttctg gaggcggttc tatggcttcc tccgaagatg ttatcaaaga
     1501 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
     1561 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
     1621 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
     1681 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
     1741 tttcaaatgg gaacgtgtta tgaacttcga agatggtggt gttgttaccg ttacccagga
     1801 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
     1861 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
     1921 gtacccggaa gatggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
     1981 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
     2041 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag attacaccat
     2101 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aa
//