LOCUS       BBa_J100214             2139 bp    DNA     linear       26-Nov-2024
DEFINITION  tCloneTet+Red fusion protein reporter with Riboswitch 10Shift
ACCESSION   BBa_J100214
VERSION     BBa_J100214.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 promoter
     stem_loop       41..127
                     /label=Riboswitch 10 Shift
     CDS             217..1410
                     /label=TetA
     CDS             1411..1458
                     /label=Linker sequence
     CDS             1459..2139
                     /label=RFP
     RBS             132..216
                     /label=BD18 C dog RBS
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac aagtgatacc agcatcgtct
       61 tgatgccctt ggcagcactt cagaaatctc tgaagtgctg ttttttttaa ttcgagctcc
      121 gtcgacagcg ggggcccaag ttcacttaaa aaggagatca acaatgaaag caattttcgt
      181 actgaaacat cttaatcatg cgacggagcg tttctaatgg gacttaaatc taacaatgcg
      241 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
      301 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
      361 gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
      421 tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
      481 tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc
      541 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      601 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
      661 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
      721 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
      781 ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
      841 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
      901 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
      961 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     1021 actggccccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     1081 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     1141 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     1201 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     1261 acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     1321 aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     1381 cgcggtgcat ggagccgggc cacctcgacc ggcggtggaa gcggcggcgg ctccggtggt
     1441 ggttctggag gcggttctat ggcttcctcc gaagatgtta tcaaagagtt catgcgtttc
     1501 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
     1561 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
     1621 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
     1681 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
     1741 cgtgttatga acttcgaaga tggtggtgtt gttaccgtta cccaggactc ctccctgcaa
     1801 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
     1861 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagat
     1921 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
     1981 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
     2041 accgacatca aactggacat cacctcccac aacgaagatt acaccatcgt tgaacagtac
     2101 gaacgtgctg aaggtcgtca ctccaccggt gcttaataa
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