LOCUS       BBa_J100215             2129 bp    DNA     linear       23-Nov-2024
DEFINITION  tCloneTet+Red fusion protein reporter with Riboswitch 4
ACCESSION   BBa_J100215
VERSION     BBa_J100215.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 promoter
     stem_loop       41..117
                     /label=Riboswitch 4
     RBS             122..206
                     /label=BD18 C dog RBS
     CDS             207..1400
                     /label=TetA
     CDS             1401..1448
                     /label=Linker
     CDS             1449..2129
                     /label=RFP
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac aagtgatacc agcatcgtct
       61 tgatgccctt ggcagcactt caaaccgaaa tttgcgcttg aagtgctgct tttttttgcg
      121 ggggcccaag ttcacttaaa aaggagatca acaatgaaag caattttcgt actgaaacat
      181 cttaatcatg cgacggagcg tttctaatgg gacttaaatc taacaatgcg ctcatcgtca
      241 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
      301 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
      361 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
      421 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
      481 tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
      541 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
      601 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
      661 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
      721 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
      781 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
      841 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
      901 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
      961 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggccccg
     1021 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     1081 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     1141 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     1201 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     1261 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     1321 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     1381 ggagccgggc cacctcgacc ggcggtggaa gcggcggcgg ctccggtggt ggttctggag
     1441 gcggttctat ggcttcctcc gaagatgtta tcaaagagtt catgcgtttc aaagttcgta
     1501 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     1561 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     1621 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     1681 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     1741 acttcgaaga tggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     1801 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     1861 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagat ggtgctctga
     1921 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     1981 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     2041 aactggacat cacctcccac aacgaagatt acaccatcgt tgaacagtac gaacgtgctg
     2101 aaggtcgtca ctccaccggt gcttaataa
//