BBa_J100217 1 BBa_J100217 tCloneTet with Riboswitch 3 2015-06-23T11:00:00Z 2015-08-31T04:08:24Z Part BBa_J119374, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ . This is a hybrid taken from part J119374, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, Tetracycline Resistance) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed. false false _578_ 0 23950 9 Not in stock false The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed. false Julia Preziosi annotation2432894 1 P5 promoter range2432894 1 1 36 annotation2432897 1 TetA range2432897 1 211 1407 annotation2432896 1 BD18 C dog RBS range2432896 1 126 210 annotation2432895 1 Riboswitch 3 range2432895 1 41 121 BBa_J100217_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacaagtgataccagcatcgtcttgatgcccttggcagcacttcatttacatactcggtaaactgaagtgctgccattttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgggacttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z