BBa_J100219 1 BBa_J100219 tCloneTet with Riboswitch 8 2015-06-23T11:00:00Z 2015-08-31T04:08:24Z Part BBa_J119374, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ . Using Golden Gate Assembly, the transcriptional riboswitch RS8 (from Wachsmuth, et al.) was ligated into the destination vector tClone Tet (J119374) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine. Based on the previous research of Wachsmuth, et al., we do not expect this to be a very effective riboswitch (small dynamic range). We will be using it as a negative control. false false _578_ 0 23950 9 Not in stock false The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed. false Julia Preziosi annotation2432902 1 P5 promoter range2432902 1 1 36 annotation2432905 1 TetA range2432905 1 202 1398 annotation2432904 1 BD18 C dog RBS range2432904 1 117 201 annotation2432903 1 Riboswitch 8 range2432903 1 41 112 BBa_J100219_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacaagtgataccagcatcgtcttgatgcccttggcagcacttcactcctagtggagtgaagtgctgttttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgggacttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z