BBa_J100222 1 BBa_J100222 Xanthine Aptamer from riboCalc with RS3, riboswitch in tCloneRed 2015-07-06T11:00:00Z 2015-08-31T04:08:24Z tCloneRed = BBa_J119361 Riboswitch 3 = J100207; Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ . This part comes from the combination of a xanthine aptamer, as determined by the Riboswitch Calculator, with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, xanthine. false false _578_ 0 23950 9 Not in stock false We had to add the aptamer to a riboswitch so that we could get the selective termination via the stem-loop behavior. We therefore did't know how the total riboswitch would fold or if it would be a successful riboswitch. Aptamer alone = GGCACGUGUAUUACCCUAGUGGUCGACGUGCC false Julia Preziosi annotation2432970 1 BD 18 C dog RBS range2432970 1 116 200 annotation2432973 1 Xanthine Riboswitch range2432973 1 41 111 annotation2432964 1 P5 promoter range2432964 1 1 36 annotation2432976 1 RFP range2432976 1 201 881 BBa_J100222_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacggcacgtgtattaccctagtggtcgacgtgcctttacatactcggtaaacggcacgtcgaccattttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z