LOCUS       BBa_J100223              947 bp    DNA     linear       09-Jan-2025
DEFINITION  Caffeine Aptamer with RS3, riboswitch in tCloneRed
ACCESSION   BBa_J100223
VERSION     BBa_J100223.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 promoter
     RBS             182..266
                     /label=BD 18 C dog RBS
     stem_loop       41..177
                     /label=Caffeine RIboswitch
     CDS             267..947
                     /label=RFP
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac ggatgtccag tcgcttgcaa
       61 tgccctttta gaccctgatg aggatcatcg gactttgtcc tgtggagtaa gtcgcgaaac
      121 ggtgaaagcc gtaggtcttt tacatactcg gtaaacagac ctacggcttt tttttttgcg
      181 ggggcccaag ttcacttaaa aaggagatca acaatgaaag caattttcgt actgaaacat
      241 cttaatcatg cgacggagcg tttctaatgg cttcctccga agatgttatc aaagagttca
      301 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg
      361 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg
      421 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt
      481 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca
      541 aatgggaacg tgttatgaac ttcgaagatg gtggtgttgt taccgttacc caggactcct
      601 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg
      661 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc
      721 cggaagatgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc
      781 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg
      841 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagattac accatcgttg
      901 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataa
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