BBa_J100223
1
BBa_J100223
Caffeine Aptamer with RS3, riboswitch in tCloneRed
2015-07-06T11:00:00Z
2015-08-31T04:08:24Z
tCloneRed = BBa_J119361
Riboswitch 3 = J100207; Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .
This part comes from the combination of a caffeine aptamer with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, caffeine.
false
false
_578_
0
23950
9
Not in stock
false
We had to add the aptamer to a riboswitch so that we could get the selective termination via the stem-loop behavior. We therefore did't know how the total riboswitch would fold or if it would be a successful riboswitch.
Aptamer alone = GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGGAUCAUCGGACUUUGUCCUGUGGAGUAAGUCGCGAAACGGUGAAAGCCGUAGGUCU
false
Julia Preziosi
annotation2432971
1
BD 18 C dog RBS
range2432971
1
182
266
annotation2432977
1
RFP
range2432977
1
267
947
annotation2432974
1
Caffeine RIboswitch
range2432974
1
41
177
annotation2432965
1
P5 promoter
range2432965
1
1
36
BBa_J100223_sequence
1
ttgacaattaatcatccggctcgtaatttatgtggacgacggatgtccagtcgcttgcaatgcccttttagaccctgatgaggatcatcggactttgtcctgtggagtaagtcgcgaaacggtgaaagccgtaggtcttttacatactcggtaaacagacctacggcttttttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z