LOCUS       BBa_J100238             2063 bp    DNA     linear       26-Nov-2024
DEFINITION  tCloneTetRed with short stuffer
ACCESSION   BBa_J100238
VERSION     BBa_J100238.1  
FEATURES             Location/Qualifiers
     promoter        1..37
                     /label=P5 promoter
     misc_feature    41..53
                     /label=Short Stuffer 
     CDS             141..1335
                     /label=TetA protein half
     misc_feature    1336..1385
                     /label=Linker sequence
     CDS             1386..2063
                     /label=RFP protein half
     misc_feature    53..140
                     /label=BD18 C Dog RBS
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac gtgatcctac agcgggggcc
       61 caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat
      121 catgcgacgg agcgtttcta atgggactta aatctaacaa tgcgctcatc gtcatcctcg
      181 gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct
      241 tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat
      301 atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc
      361 gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga
      421 ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
      481 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
      541 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
      601 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
      661 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga
      721 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
      781 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
      841 tctgggtcat tttcggcgag gatcgctttc gctggagcgc gacgatgatc ggcctgtcgc
      901 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
      961 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     1021 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     1081 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     1141 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac
     1201 cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     1261 ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     1321 gagccacctc gaccggcggt ggaagcggcg gcggctccgg tggtggttct ggaggcggtt
     1381 ctatggcttc ctccgaagat gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
     1441 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
     1501 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
     1561 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
     1621 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
     1681 aagatggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
     1741 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
     1801 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agatggtgct ctgaaaggtg
     1861 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
     1921 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
     1981 acatcacctc ccacaacgaa gattacacca tcgttgaaca gtacgaacgt gctgaaggtc
     2041 gtcactccac cggtgcttaa taa
//