BBa_J100238 1 BBa_J100238 tCloneTetRed with short stuffer 2015-11-09T12:00:00Z 2015-11-10T01:20:24Z The part comes from J119361 tCloneRed and J119140 TetA. The linker sequence was developed from K598018 tetA+GFP fused protein by Qingyang XIAO. The short stuffer sequence is 5' GTGATCCTACA. This is the tConeTetRed insert (part BBa_J119386) in pUC-BR plasmid. The insert has had the P2 promoter and GFP gene removed by digestion with BsaI and has had a short stuffer sequence (11bp) ligated in its place. This was used to test the effectiveness of tConeTetRed without the presence of a riboswitch, so the presence of the Tet-A RFP fusion protein is directly controlled by the P5 promoter. This was tested in JM109 E. coli cells and produces measurable fluorescence when grown in the presence of Tetracycline in a range of concentrations from 10 to 50 ug/mL. false false _578_ 23978 23978 9 false When tCloneTetRed is used to test riboswitches, there are approximately 80 bases added between the promoter and ribosome binding site. To test the efficiency of the system without the increased distance between the promoter and RBS, we synthesized this short stuffer sequence to minimize the distance and test the 'natural' activity of the system. false Nicholas Elder annotation2478410 1 TetA protein half range2478410 1 141 1335 annotation2478409 1 Short Stuffer range2478409 1 41 53 annotation2478408 1 P5 promoter range2478408 1 1 37 annotation2478411 1 Linker sequence range2478411 1 1336 1385 annotation2478412 1 RFP protein half range2478412 1 1386 2063 annotation2478418 1 BD18 C Dog RBS range2478418 1 53 140 BBa_J100238_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacgtgatcctacagcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgggacttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggatcgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgagccacctcgaccggcggtggaagcggcggcggctccggtggtggttctggaggcggttctatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z