LOCUS       BBa_J100239             2128 bp    DNA     linear       14-May-2024
DEFINITION  tCloneTetRed with scrambled riboswitch
ACCESSION   BBa_J100239
VERSION     BBa_J100239.1  
FEATURES             Location/Qualifiers
     promoter        1..37
                     /label=P5 promoter
     misc_feature    41..117
                     /label=Scrambled riboswitch
     CDS             206..1400
                     /label=TetA protein half
     misc_feature    1401..1448
                     /label=Linker Sequence 
     CDS             1449..2128
                     /label=RFP protein half
     misc_feature    119..205
                     /label=BD18 C Dog RBS
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tattgagcgc gctcccgccc
       61 taatcctgtt agttcaagtg ggaggagtgg cccagtcaca ctagttaatc ctaggagcgg
      121 gggcccaagt tcacttaaaa aggagatcaa caatgaaagc aattttcgta ctgaaacatc
      181 ttaatcatgc gacggagcgt ttctaatggg acttaaatct aacaatgcgc tcatcgtcat
      241 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      301 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      361 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      421 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      481 ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
      541 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      601 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      661 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
      721 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
      781 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      841 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      901 agcgctctgg gtcattttcg gcgaggatcg ctttcgctgg agcgcgacga tgatcggcct
      961 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1021 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1081 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1141 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1201 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1261 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1321 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1381 gagccgagcc acctcgaccg gcggtggaag cggcggcggc tccggtggtg gttctggagg
     1441 cggttctatg gcttcctccg aagatgttat caaagagttc atgcgtttca aagttcgtat
     1501 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
     1561 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     1621 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     1681 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1741 cttcgaagat ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1801 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1861 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagatg gtgctctgaa
     1921 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1981 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     2041 actggacatc acctcccaca acgaagatta caccatcgtt gaacagtacg aacgtgctga
     2101 aggtcgtcac tccaccggtg cttaataa
//