BBa_J100239 1 BBa_J100239 tCloneTetRed with scrambled riboswitch 2015-11-09T12:00:00Z 2015-11-10T01:21:57Z The part J119386 comes from J119361 tCloneRed and J119140 TetA. The linker sequence was developed from K598018 tetA+GFP fused protein by Qingyang XIAO. The scrambled riboswitch sequence is 5' TATTGAGCGCGCTCCCGCCCTAATCCTGTTAGTTCAAGTGGGAGGAGTGGCCCAGTCACACTAGTTAATCCTAGGA. This is based on the tConeTetRed insert (part BBa_J119386). The insert had the P2 promoter and GFP gene removed by digestion with BsaI and has had a scrambled sequence of a known riboswitch ligated in its place. This was used to test the effectiveness of tConeTetRed without the presence of a riboswitch, but with the added genetic distance of a functional riboswitch between the promoter and RBS. This was tested in JM109 E. coli cells and produces measurable fluorescence when grown in the presence of Tetracycline (20 ug/mL). false false _578_ 23978 23978 9 false When tCloneTetRed is used to test riboswitches, there are approximately 80 bases added between the promoter and ribosome binding site. To test the efficiency of the system with the increased distance between the promoter and RBS, we synthesized this scrambled riboswitch sequence to insert into the part to test the activity of the system without the regulatory capabilities. false Nicholas Elder annotation2478414 1 Scrambled riboswitch range2478414 1 41 117 annotation2478413 1 P5 promoter range2478413 1 1 37 annotation2478416 1 Linker Sequence range2478416 1 1401 1448 annotation2478415 1 TetA protein half range2478415 1 206 1400 annotation2478419 1 BD18 C Dog RBS range2478419 1 119 205 annotation2478417 1 RFP protein half range2478417 1 1449 2128 BBa_J100239_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgactattgagcgcgctcccgccctaatcctgttagttcaagtgggaggagtggcccagtcacactagttaatcctaggagcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgggacttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggatcgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgagccacctcgaccggcggtggaagcggcggcggctccggtggtggttctggaggcggttctatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z