LOCUS       BBa_J100251             1718 bp    DNA     linear       23-Dec-2024
DEFINITION  pClone Cherry
ACCESSION   BBa_J100251
VERSION     BBa_J100251.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP reverse 
     RBS             727..738
                     /label=B0030 RBS reverse
     misc_feature    755..758
                     /label=Sticky end
     misc_feature    760..765
                     /label=BsaI left
     promoter        766..836
                     /label=pLaclQ1 reverse
     misc_feature    837..842
                     /label=BsaI right 
     misc_feature    844..847
                     /label=Sticky end
     RBS             860..944
                     /label=BD18 bicistroin RBS
     CDS             945..1712
                     /label=mCherry 
     misc_feature    1713..1718
                     /label=double stop
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactg agaccccggg cgctatcatg
      781 ccataccgcg aaaggttttg caccattcga tggtgtcaac gtaaatgcat gccgctggtc
      841 tctgcgggaa gacaactagg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      901 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggga tcctcgcatc
      961 atcatcacca ccattcctca ggtttagtcc ctcgcgggag ccatatggta tcgaaagggg
     1021 aagaggacaa tatggcaatt atcaaggagt tcatgcgttt taaggttcat atggagggga
     1081 gtgtaaacgg ccacgaattc gagatcgaag gtgaagggga agggcgtcca tatgagggta
     1141 cacagactgc taagttaaag gtcacgaagg gcggcccttt acctttcgca tgggatattc
     1201 tgtcgcccca gtttatgtat ggctcgaagg cttatgtaaa acacccggcg gatattcctg
     1261 actatttaaa actgtcattc ccagaaggtt ttaagtggga gcgcgtcatg aacttcgagg
     1321 atggaggcgt cgtaacagtt acccaggatt catcgttgca agacggggag ttcatttaca
     1381 aagttaaact tcgtggcacg aactttccta gtgacggacc tgtaatgcaa aagaagacca
     1441 tggggtggga ggcttcctcg gagcgcatgt accccgagga cggcgctttg aaaggggaga
     1501 tcaaacaacg cttgaaattg aaagatggag ggcattatga tgcagaggtg aaaaccacat
     1561 acaaggctaa gaagccagtg caactgccgg gtgcatacaa tgttaatatc aaactggata
     1621 ttacctctca caatgaggac tatacgatcg tcgaacagta tgagcgcgcc gaaggacgcc
     1681 attccacagg cggtatggat gaactttaca agtaataa
//