##gff-version 3
##sequence-region BBa_J100251 1 1718
BBa_J100251	.	CDS	1	720	.	+	0	ID=annotation2478518;Name=GFP reverse 
BBa_J100251	.	sequence_feature	844	847	.	+	0	ID=annotation2478524;Name=Sticky end
BBa_J100251	.	ribosome_entry_site	860	944	.	+	0	ID=annotation2478525;Name=BD18 bicistroin RBS
BBa_J100251	.	ribosome_entry_site	727	738	.	+	0	ID=annotation2478519;Name=B0030 RBS reverse
BBa_J100251	.	promoter	766	836	.	+	0	ID=annotation2478522;Name=pLaclQ1 reverse
BBa_J100251	.	sequence_feature	760	765	.	+	0	ID=annotation2478521;Name=BsaI left
BBa_J100251	.	CDS	945	1712	.	+	0	ID=annotation2478526;Name=mCherry 
BBa_J100251	.	sequence_feature	837	842	.	+	0	ID=annotation2478523;Name=BsaI right 
BBa_J100251	.	stop_codon	1713	1718	.	+	0	ID=annotation2478527;Name=double stop
BBa_J100251	.	sequence_feature	755	758	.	+	0	ID=annotation2478520;Name=Sticky end
>BBa_J100251
ttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctc
ttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaa
ttggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaacttt
gattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatg
tttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcac
gtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaa
gtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccat
ggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccac
tgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcc
tttacgcatctagtatttctcctctttaatactagaggtcttccgactgagaccccgggcgctatcatgccataccgcg
aaaggttttgcaccattcgatggtgtcaacgtaaatgcatgccgctggtctctgcgggaagacaactaggggcccaagt
tcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgg
gatcctcgcatcatcatcaccaccattcctcaggtttagtccctcgcgggagccatatggtatcgaaaggggaagagga
caatatggcaattatcaaggagttcatgcgttttaaggttcatatggaggggagtgtaaacggccacgaattcgagatc
gaaggtgaaggggaagggcgtccatatgagggtacacagactgctaagttaaaggtcacgaagggcggccctttacctt
tcgcatgggatattctgtcgccccagtttatgtatggctcgaaggcttatgtaaaacacccggcggatattcctgacta
tttaaaactgtcattcccagaaggttttaagtgggagcgcgtcatgaacttcgaggatggaggcgtcgtaacagttacc
caggattcatcgttgcaagacggggagttcatttacaaagttaaacttcgtggcacgaactttcctagtgacggacctg
taatgcaaaagaagaccatggggtgggaggcttcctcggagcgcatgtaccccgaggacggcgctttgaaaggggagat
caaacaacgcttgaaattgaaagatggagggcattatgatgcagaggtgaaaaccacatacaaggctaagaagccagtg
caactgccgggtgcatacaatgttaatatcaaactggatattacctctcacaatgaggactatacgatcgtcgaacagt
atgagcgcgccgaaggacgccattccacaggcggtatggatgaactttacaagtaataa