BBa_J100252 1 BBa_J100252 tCloneTet with RS10shift 2016-03-23T12:00:00Z 2016-03-23T09:55:23Z Synthesized riboswitch and used J119367. Started with tCloneTet (J119367) and used Golden Gate Assembly to remove GFP reporter and put transcriptional terminator riboswitch RS10shift (RS10s). RS10s was taken from the Wachsmuth et. al. 2013 NAR vol. 41 p. 2541. This riboswitch works and is responsive to theophylline (2.5mM). false false _578_ 23979 23979 9 false Riboswitch cannot have internal Bsa1 restriction site. false Helen Webster annotation2478528 1 P5 Promoter range2478528 1 1 36 annotation2478529 1 RS10shift Riboswitch range2478529 1 42 128 annotation2478531 1 RFP protein half range2478531 1 219 899 annotation2478530 1 BD18 C Dog RBS range2478530 1 134 218 BBa_J100252_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgactaagtgataccagcatcgtcttgatgcccttggcagcacttcagaaatctctgaagtgctgttttttttaattcgagctccgtcgacatgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z