LOCUS       BBa_J100253             1669 bp    DNA     linear       22-Dec-2024
DEFINITION  This is a modification of pClone Cherry (J100251)
ACCESSION   BBa_J100253
VERSION     BBa_J100253.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP reverse
     RBS             727..738
                     /label=B0030 RBS reverse
     promoter        759..794
                     /label=P5
     misc_feature    795..798
                     /label=Sticky end
     RBS             811..895
                     /label=BD18 bicistroin RBS
     CDS             896..1663
                     /label=mCherry 
     misc_feature    755..758
                     /label=Sticky end
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactt gacaattaat catccggctc
      781 gtaatttatg tggagcggga agacaactag gggcccaagt tcacttaaaa aggagatcaa
      841 caatgaaagc aattttcgta ctgaaacatc ttaatcatgc gacggagcgt ttctaatggg
      901 atcctcgcat catcatcacc accattcctc aggtttagtc cctcgcggga gccatatggt
      961 atcgaaaggg gaagaggaca atatggcaat tatcaaggag ttcatgcgtt ttaaggttca
     1021 tatggagggg agtgtaaacg gccacgaatt cgagatcgaa ggtgaagggg aagggcgtcc
     1081 atatgagggt acacagactg ctaagttaaa ggtcacgaag ggcggccctt tacctttcgc
     1141 atgggatatt ctgtcgcccc agtttatgta tggctcgaag gcttatgtaa aacacccggc
     1201 ggatattcct gactatttaa aactgtcatt cccagaaggt tttaagtggg agcgcgtcat
     1261 gaacttcgag gatggaggcg tcgtaacagt tacccaggat tcatcgttgc aagacgggga
     1321 gttcatttac aaagttaaac ttcgtggcac gaactttcct agtgacggac ctgtaatgca
     1381 aaagaagacc atggggtggg aggcttcctc ggagcgcatg taccccgagg acggcgcttt
     1441 gaaaggggag atcaaacaac gcttgaaatt gaaagatgga gggcattatg atgcagaggt
     1501 gaaaaccaca tacaaggcta agaagccagt gcaactgccg ggtgcataca atgttaatat
     1561 caaactggat attacctctc acaatgagga ctatacgatc gtcgaacagt atgagcgcgc
     1621 cgaaggacgc cattccacag gcggtatgga tgaactttac aagtaataa
//