BBa_J100253 1 BBa_J100253 This is a modification of pClone Cherry (J100251) 2016-04-04T11:00:00Z 2016-04-24T02:50:16Z pClone Cherry (J100251) This is a modification of pClone Cherry (J100251). GFP promoter pLaclQ1 was removed and a BD18 bicistroin RBS promoter was put in its place false false _578_ 18865 18865 9 false Make sure the sticky ends aligned with the ligating promoter. false Grace Harper annotation2478614 1 GFP reverse range2478614 1 1 720 annotation2478616 1 P5 range2478616 1 759 794 annotation2478619 1 mCherry range2478619 1 896 1663 annotation2478618 1 BD18 bicistroin RBS range2478618 1 811 895 annotation2478620 1 Sticky end range2478620 1 755 758 annotation2478615 1 B0030 RBS reverse range2478615 1 727 738 annotation2478617 1 Sticky end range2478617 1 795 798 BBa_J100253_sequence 1 ttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcatctagtatttctcctctttaatactagaggtcttccgacttgacaattaatcatccggctcgtaatttatgtggagcgggaagacaactaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgggatcctcgcatcatcatcaccaccattcctcaggtttagtccctcgcgggagccatatggtatcgaaaggggaagaggacaatatggcaattatcaaggagttcatgcgttttaaggttcatatggaggggagtgtaaacggccacgaattcgagatcgaaggtgaaggggaagggcgtccatatgagggtacacagactgctaagttaaaggtcacgaagggcggccctttacctttcgcatgggatattctgtcgccccagtttatgtatggctcgaaggcttatgtaaaacacccggcggatattcctgactatttaaaactgtcattcccagaaggttttaagtgggagcgcgtcatgaacttcgaggatggaggcgtcgtaacagttacccaggattcatcgttgcaagacggggagttcatttacaaagttaaacttcgtggcacgaactttcctagtgacggacctgtaatgcaaaagaagaccatggggtgggaggcttcctcggagcgcatgtaccccgaggacggcgctttgaaaggggagatcaaacaacgcttgaaattgaaagatggagggcattatgatgcagaggtgaaaaccacatacaaggctaagaagccagtgcaactgccgggtgcatacaatgttaatatcaaactggatattacctctcacaatgaggactatacgatcgtcgaacagtatgagcgcgccgaaggacgccattccacaggcggtatggatgaactttacaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z