LOCUS       BBa_J100254             1576 bp    DNA     linear       06-Dec-2025
DEFINITION  Part J119137 + P5
ACCESSION   BBa_J100254
VERSION     BBa_J100254.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP reverse
     RBS             727..738
                     /label=B0030 RBS reverse
     misc_feature    755..758
                     /label=Sticky end 
     misc_feature    795..798
                     /label=Sticky end
     RBS             811..895
                     /label=BD18 bicistroin RBS
     CDS             896..1576
                     /label=RFP
     promoter        759..794
                     /label=P5 Promoter
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactt gacaattaat catccggctc
      781 gtaatttatg tggagcggga agacaactag gggcccaagt tcacttaaaa aggagatcaa
      841 caatgaaagc aattttcgta ctgaaacatc ttaatcatgc gacggagcgt ttctaatggc
      901 ttcctccgaa gatgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      961 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     1021 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     1081 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     1141 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagatgg
     1201 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1261 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1321 ttgggaagct tccaccgaac gtatgtaccc ggaagatggt gctctgaaag gtgaaatcaa
     1381 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1441 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1501 ctcccacaac gaagattaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1561 caccggtgct taataa
//