LOCUS       BBa_J100258             1800 bp    DNA     linear       26-Dec-2024
DEFINITION  B Nitrate Biosensor (both)
ACCESSION   BBa_J100258
VERSION     BBa_J100258.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP Reverse
     promoter        755..1021
                     /label=fixed both narG promoter 
     CDS             1119..1800
                     /label=RFP
     RBS             1033..1118
                     /label=BD18 bicistron RBS
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactg ttatatggtt tatttcgccg
      781 gatttcatta agagccatta atatgttacc catggggaat actccttaat acccatctgc
      841 ataaaaatct taatagttta aataactaca ggtataaaac gtcttaattt acagtctgtt
      901 atgtggtggc tgatacccat cttaaagggg tatcttagtt tcatccccat cactttcatc
      961 cccatcactc ttgatcgtta tcaattccca cgctgtttca gagcgttacc ttgcccttgc
     1021 gggaagacaa ctaggggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt
     1081 cgtactgaaa catcttaatc atgcgacgga gcgtttctaa tggcttcctc cgaagatgtt
     1141 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
     1201 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
     1261 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
     1321 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
     1381 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag atggtggtgt tgttaccgtt
     1441 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
     1501 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
     1561 gaacgtatgt acccggaaga tggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
     1621 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
     1681 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagat
     1741 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
//