BBa_J100258 1 BBa_J100258 B Nitrate Biosensor (both) 2016-05-15T11:00:00Z 2016-07-12T12:31:18Z E. coli K-12 MG1655 Transcription Unit: narGHJI This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by disrupting both the left and right Fis binding site, where an inhibitory regulator binds; moreover,since there is an overlap between the activator and inhibitor binding site, we had to make sure the activator can still bind on the same site. The modified promtoer is inserted in to pClone Red (J119137). Nitrate induces the promoter, so RFP is expressed when nitrate is present. false false _578_ 23989 23989 9 false There is a deletion at BD18 bicistron RBS. false Shuk Hang Li annotation2479673 1 BD18 bicistron RBS range2479673 1 1033 1118 annotation2479666 1 GFP Reverse range2479666 1 1 720 annotation2479669 1 fixed both narG promoter range2479669 1 755 1021 annotation2479672 1 RFP range2479672 1 1119 1800 BBa_J100258_sequence 1 ttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcatctagtatttctcctctttaatactagaggtcttccgactgttatatggtttatttcgccggatttcattaagagccattaatatgttacccatggggaatactccttaatacccatctgcataaaaatcttaatagtttaaataactacaggtataaaacgtcttaatttacagtctgttatgtggtggctgatacccatcttaaaggggtatcttagtttcatccccatcactttcatccccatcactcttgatcgttatcaattcccacgctgtttcagagcgttaccttgcccttgcgggaagacaactaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z