LOCUS       BBa_J100259             1641 bp    DNA     linear       22-Nov-2024
DEFINITION  DL Nitrate Biosensor
ACCESSION   BBa_J100259
VERSION     BBa_J100259.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP Reverse
     promoter        755..863
                     /label=DL narG promoter 
     CDS             961..1641
                     /label=RFP
     RBS             876..960
                     /label=BD18 bicistron RBS
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactt atcctaaagg ggtatcttag
      781 gaatttactt tatttttcat ccccatcact cttgatcgtt atcaattccc acgctgtttc
      841 agagcgttac cttgcccttg cgggaagaca actaggggcc caagttcact taaaaaggag
      901 atcaacaatg aaagcaattt tcgtactgaa acatcttaat catgcgacgg agcgtttcta
      961 atggcttcct ccgaagatgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
     1021 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
     1081 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
     1141 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
     1201 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
     1261 gatggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
     1321 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
     1381 atgggttggg aagcttccac cgaacgtatg tacccggaag atggtgctct gaaaggtgaa
     1441 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
     1501 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
     1561 atcacctccc acaacgaaga ttacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
     1621 cactccaccg gtgcttaata a
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