LOCUS       BBa_J100260             1642 bp    DNA     linear       22-Nov-2024
DEFINITION  DB Nitrate Biosensor
ACCESSION   BBa_J100260
VERSION     BBa_J100260.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP Reverse
     promoter        755..864
                     /label=DB narG promoter
     CDS             962..1642
                     /label=RFP
     RBS             877..961
                     /label=BD18 bicistron RBS
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactt atcctaaagg ggtatcttag
      781 tttcatcccc atcactttca tccccatcac tcttgatcgt tatcaattcc cacgctgttt
      841 cagagcgtta ccttgccctt gcgggaagac aactaggggc ccaagttcac ttaaaaagga
      901 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgcgacg gagcgtttct
      961 aatggcttcc tccgaagatg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     1021 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     1081 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     1141 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     1201 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     1261 agatggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     1321 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     1381 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gatggtgctc tgaaaggtga
     1441 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     1501 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     1561 catcacctcc cacaacgaag attacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     1621 tcactccacc ggtgcttaat aa
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