BBa_J100262 1 BBa_J100262 rClone Red with Riboswitch E 2016-06-07T11:00:00Z 2016-06-08T10:13:19Z The sequence for riboswitch E was taken from the Gallivan et al. paper "A family of synthetic riboswitches adopts a kinetic trapping mechanism." The rClone Red plasmid used in GGA is part BBa_J119384. This part is a modified form of the rClone Red plasmid (part BBa_J119384) that replaced the GFP section flanked by BsaI sites with a theophylline riboswitch, labeled riboswitch E, by Golden Gate Assembly (GGA). In the presence of theophylline, the cells should fluoresce red. In the absence of theophylline, the cells should not fluoresce. false false _578_ 28832 28832 9 false / false Zachary Shaver annotation2480073 1 Riboswitch E range2480073 1 41 94 annotation2480074 1 Red Fluorescent Protein range2480074 1 101 781 annotation2480072 1 P5 promoter range2480072 1 1 36 BBa_J100262_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggaggtaacgcggagatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z