LOCUS BBa_J100264 4722 bp DNA linear 23-Dec-2024 DEFINITION pJC156c2 (Carlson et al. 2014) ACCESSION BBa_J100264 VERSION BBa_J100264.1 FEATURES Location/Qualifiers misc_feature 1..589 /label=ColE1 Origin of Replication CDS 1017..1208 /label=Rop promoter 1368..1524 /label=PlacZ RBS 1540..1554 /label=SD8 RBS CDS 1555..1872 /label=geneIII (C83) misc_feature 1872..2591 /label=Venus YFP CDS 2664..3445 /label=aadA promoter 3456..3540 /label=aadA promoter CDS 3557..4639 /label=lacI promoter 4663..4706 /label=lacIq ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accacctctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag 841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg 1081 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc 1141 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt 1201 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg 1261 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacaagagga catccggtca 1321 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttgctgagg agacttaggg 1381 accctggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 1441 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 1501 tgtgagcgga taacaatttc acacacctgc aggtgcagta aggaggaaaa aaaaatgaaa 1561 aaattattat tcgcaattcc tttagttgtt cctttctatt ctcactccgc tgaaactgtt 1621 tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa 1681 tatttacctt ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cgctggtaaa 1741 ccttacgagt tcagtatcga ctgcgataag atcaacctgt tccgcggtgt ctttgcgttt 1801 cttttatatg ttgccacctt tatgtatgta ttttctacgt ttgctaacat actgcgtaat 1861 aaggagtctt aatggtcagt aaaggagaag aacttttcac tggagttgtc ccaattcttg 1921 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg 1981 atgcaacata cggaaaactt acccttaaac tgatttgcac tactggaaaa ctacctgttc 2041 catggccaac acttgtcact actctgggct atggtctgca atgctttgcc agatacccag 2101 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa 2161 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag 2221 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca 2281 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc accgcagaca 2341 aacaaaagaa tggaatcaaa gccaacttca aaattagaca caacattgaa gatggaggcg 2401 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac 2461 cagacaacca ttacctgtcc taccaatctg ccctttcgaa agatcccaac gaaaagagag 2521 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac 2581 tatacaaata aacttaatta acggcactaa atataatgac cctcttgata acccaagagg 2641 gcatttttta atgcccatgg cgtttatttg ccgactacct tggtgatctc gcctttcacg 2701 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca 2761 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt 2821 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg 2881 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat 2941 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt 3001 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata 3061 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc 3121 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc 3181 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt 3241 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta 3301 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa 3361 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt 3421 tgagtcgata cttcggcgat caccgcttcc ctcatactct tcctttttca atattattga 3481 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 3541 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 3601 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt 3661 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga 3721 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg 3781 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat 3841 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat 3901 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt 3961 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc 4021 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc 4081 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg 4141 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa 4201 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg 4261 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac 4321 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg 4381 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg 4441 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt 4501 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg 4561 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt 4621 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg 4681 ccataccgcg aaaggttttg caccataggc caaataggcc gt //