LOCUS BBa_J100266 4105 bp DNA linear 19-Apr-2024 DEFINITION pJC173g-SD8_AP-neg (Carlson et al. 2014) ACCESSION BBa_J100266 VERSION BBa_J100266.1 FEATURES Location/Qualifiers misc_feature 1..799 /label=pUC Origin of Replication misc_feature 815..1239 /label=rrnB1 transcriptional terminator promoter 1264..1283 /label=T7 promoter RBS 1305..1319 /label=SD8 RBS CDS 1320..2414 /label=geneIII neg misc_feature 2414..3133 /label=Venus YFP CDS 3213..4004 /label=aadA spectinomycin resistance promoter 4005..4089 /label=aadA promoter ORIGIN 1 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 61 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 781 gaggaagcgg aagagcgcca agaggacatc cggtggctgt tttggcggat gagagaagat 841 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc 901 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg 961 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa 1021 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1081 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc 1141 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg 1201 ccatcctgac ggatggcctt tttgcgtttc tacaaactcg ctgaggagac ttagggaccc 1261 tactaatacg actcactata gggagaaaga cctgcaggtg cagtaaggag gaaaaaaaaa 1321 tgaaaaaatt attattcgca attcctttag ttgttccttt ctattctcac tccgctgaaa 1381 ctgttcatca ccatcaccat cacgctgaaa ctgttgaaag ttgtttagca aaaccccata 1441 cagaaaattc atttactaac gtctggaaag acgacaaaac tttagatcgt tacgctaact 1501 atgagggctg tctgtggaat gctacaggcg ttgtagtttg tactggtgac gaaactcagt 1561 gttacggtac atgggttcct attgggcttg ctatccctga aaatgagggt ggtggctctg 1621 agggtggcgg ttctgagggt ggcggttctg agggtggcgg tactaaacct cctgagtacg 1681 gtgatacacc tattccgggc tatacttata tcaaccctct cgacggcact tatccgcctg 1741 gtactgagca aaaccccgct aatcctaatc cttctcttga ggagtctcag cctcttaata 1801 ctttcatgtt tcagaataat aggttccgaa ataggcaggg ggcattaact gtttatacgg 1861 gcactgttac tcaaggcact gaccccgtta aaacttatta ccagtacact cctgtatcat 1921 caaaagccat gtatgacgct tactggaacg gtaaattcag agactgcgct ttccattctg 1981 gctttaatga ggatccattc gtttgtgaat atcaaggcca atcgtctgac ctgcctcaac 2041 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg 2101 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct 2161 cttcccaaat ggctcaagtc ggtgacggtg ataattcacc tttaatgaat aatttccgtc 2221 aatatttacc ttccctccct caatcggttg aatgtcgccc ttttgtcttt ggcgctggta 2281 aaccttacga gttcagtatc gactgcgata agatcaacct gttccgcggt gtctttgcgt 2341 ttcttttata tgttgccacc tttatgtatg tattttctac gtttgctaac atactgcgta 2401 ataaggagtc ttaatggtca gtaaaggaga agaacttttc actggagttg tcccaattct 2461 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg 2521 tgatgcaaca tacggaaaac ttacccttaa actgatttgc actactggaa aactacctgt 2581 tccatggcca acacttgtca ctactctggg ctatggtctg caatgctttg ccagataccc 2641 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga 2701 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga 2761 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa 2821 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcaccgcaga 2881 caaacaaaag aatggaatca aagccaactt caaaattaga cacaacattg aagatggagg 2941 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt 3001 accagacaac cattacctgt cctaccaatc tgccctttcg aaagatccca acgaaaagag 3061 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga 3121 actatacaaa taaacttaat taacggcact cctcagcaaa tataatgacc ctcttgataa 3181 cccaagaggg cattttttaa tgcccatggc gtttatttgc cgactacctt ggtgatctcg 3241 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct 3301 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg 3361 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg 3421 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc 3481 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga 3541 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc 3601 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca 3661 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg 3721 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa 3781 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg 3841 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag 3901 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc 3961 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatactctt cctttttcaa 4021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 4081 tagaaaaata ggccaaatag gccgt //