LOCUS BBa_J100267 4269 bp DNA linear 22-Nov-2024 DEFINITION pJC174c-R5 (Carlson et al. 2014) ACCESSION BBa_J100267 VERSION BBa_J100267.1 FEATURES Location/Qualifiers misc_feature 1..589 /label=ColE1 Origin of Replication CDS 1017..1208 /label=Rop promoter 1388..1407 /label=T3 Promoter primer_bind 1389..1408 /label=T3 misc_feature 1427..1483 /label=Theophylline Riboswitch 12.1 CDS 1484..2578 /label=geneIII neg misc_feature 2578..3297 /label=Venus YFP CDS 3377..4168 /label=aadA promoter 4169..4253 /label=aadA promoter ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accacctctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag 841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg 1081 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc 1141 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt 1201 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg 1261 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacaagagga catccggtca 1321 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttgctgagg agacttaggg 1381 accctacaat taaccctcac taaagggaga aaggctaata ggtaccggtg ataccagcat 1441 cgtcttgatg cccttggcag caccctgcta aggtaacaac aagatgaaaa aattattatt 1501 cgcaattcct ttagtggtgc ctttctattc tcactccgct gaaactgttc atcaccatca 1561 ccatcacgct gaaactgttg aaagttgttt agcaaaaccc catacagaaa attcatttac 1621 taacgtctgg aaagacgaca aaactttaga tcgttacgct aactatgagg gctgtctgtg 1681 gaatgctaca ggcgttgtag tttgtactgg tgacgaaact cagtgttacg gtacatgggt 1741 tcctattggg cttgctatcc ctgaaaatga gggtggtggc tctgagggtg gcggttctga 1801 gggtggcggt tctgagggtg gcggtactaa acctcctgag tacggtgata cacctattcc 1861 gggctatact tatatcaacc ctctcgacgg cacttatccg cctggtactg agcaaaaccc 1921 cgctaatcct aatccttctc ttgaggagtc tcagcctctt aatactttca tgtttcagaa 1981 taataggttc cgaaataggc agggggcatt aactgtttat acgggcactg ttactcaagg 2041 cactgacccc gttaaaactt attaccagta cactcctgta tcatcaaaag ccatgtatga 2101 cgcttactgg aacggtaaat tcagagactg cgctttccat tctggcttta atgaggatcc 2161 attcgtttgt gaatatcaag gccaatcgtc tgacctgcct caacctcctg tcaatgctgg 2221 cggcggctct ggtggtggtt ctggtggcgg ctctgagggt ggtggctctg agggtggcgg 2281 ttctgagggt ggcggctctg agggaggcgg ttccggtggt ggctcttccc aaatggctca 2341 agtcggtgac ggtgataatt cacctttaat gaataatttc cgtcaatatt taccttccct 2401 ccctcaatcg gttgaatgtc gcccttttgt ctttggcgct ggtaaacctt acgagttcag 2461 tatcgactgc gataagatca acctgttccg cggtgtcttt gcgtttcttt tatatgttgc 2521 cacctttatg tatgtatttt ctacgtttgc taacatactg cgtaataagg agtcttaatg 2581 gtcagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 2641 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga 2701 aaacttaccc ttaaactgat ttgcactact ggaaaactac ctgttccatg gccaacactt 2761 gtcactactc tgggctatgg tctgcaatgc tttgccagat acccagatca tatgaaacag 2821 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc 2881 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt 2941 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa 3001 ttggaataca actataactc acacaatgta tacatcaccg cagacaaaca aaagaatgga 3061 atcaaagcca acttcaaaat tagacacaac attgaagatg gaggcgttca actagcagac 3121 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 3181 ctgtcctacc aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt 3241 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataaact 3301 taattaacgg cactcctcag caaatataat gaccctcttg ataacccaag agggcatttt 3361 ttaatgccca tggcgtttat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga 3421 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag 3481 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 3541 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 3601 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 3661 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 3721 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 3781 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 3841 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 3901 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 3961 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 4021 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 4081 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 4141 atacttcggc gatcaccgct tccctcatac tcttcctttt tcaatattat tgaagcattt 4201 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataggccaa 4261 ataggccgt //