LOCUS BBa_J100268 8529 bp DNA linear 27-Dec-2024 DEFINITION pJC184d5 ACCESSION BBa_J100268 VERSION BBa_J100268.1 FEATURES Location/Qualifiers promoter 17..114 /label=cat (CmR) promoter misc_feature 115..774 /label=cat (CmR) pJC184d5 CDS 881..1759 /label=araC promoter 1910..2062 /label=pBAD CDS 2120..2851 /label=dnaQ926 CDS 2889..3236 /label=umuD' CDS 3239..4519 /label=umuC CDS 4583..5644 /label=recA730 misc_feature 5662..6086 /label=rrnB1 transcriptional terminator misc_feature 6103..6232 /label=psp promoter 6233..6251 /label=tetR binding site (175h) RBS 6307..6321 /label=SD8 RBS CDS 6322..7596 /label=geneIII misc_feature 7753..8491 /label=CloDF13 ORIGIN 1 acggcctatt tggcctggca cgtaagaggt tccaactttc accataatga aataagatca 61 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag 121 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 181 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 241 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 301 cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg 361 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 421 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 481 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 541 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 601 atggacaact tcttcgcccc cgttttcact atgggcaaat attatacgca aggcgacaag 661 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc 721 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcca 781 tgggcattaa aaaatgccct cttgggttat caagagggtc attatatttc gcaaaaaacc 841 ccgcttcggc ggggtttttt cgcagtgccg ttaattaagt ttatgacaac ttgacggcta 901 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt 961 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg 1021 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc 1081 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg 1141 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc 1201 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa 1261 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat 1321 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct 1381 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc 1441 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct 1501 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc 1561 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga 1621 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct 1681 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat 1741 cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa accaattgtc 1801 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaacccaac 1861 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa 1921 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg 1981 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct 2041 ttttatcgca actctctact gtttctccat acccgttttt ttggacgcgt acaactcaag 2101 tctgacataa atgaccgcta tgagcactgc aattacacgc cagatcgttc tcgctaccgc 2161 aaccaccggt atgaaccaga ttggtgcgca ctatgaaggc cacaagatca ttgagattgg 2221 tgccgttgaa gtggtgaacc gtcgcctgac gggcaataac ttccatgttt atctcaaacc 2281 cgatcggctg gtggatccgg aagcctttgg cgtacatggt attgccgatg aatttttgct 2341 cgataagccc acgtttgccg aagtagccga tgagttcatg gactatattc gcggcgcgga 2401 gttggtgatc cataacgcag cgttcgatat cggctttatg gactacgagt tttcgttgct 2461 taagcgcgat attccgaaga ccaatacttt ctgtaaggtc accgatagcc ttgcggtggc 2521 gaggaaaatg tttcccggta agcgcaacag cctcgatgcg ttatgtgctc gctacgaaat 2581 agataacagt aaacgaacgc tgcacggggc attactcgat gcccagatcc ttgcggaagt 2641 ttatctggcg atgaccggtg gtcaaacgtc gatggctttt gcgatggaag gagagacaca 2701 acagcaacaa ggtgaagcaa caattcagcg cattgtacgt caggcaagta agttacgcgt 2761 tgtttttgcg acagatgaag agattgcagc tcatgaagcc cgtctcgatc tggtgcagaa 2821 gaaaggcgga agttgcctct ggcgagcata aaccggtatg cctcacacag gaaacagaat 2881 tcattaccat gggctttcct tcaccggcag cagattacgt tgaacagcgc atcgatctga 2941 atcaactgtt gatccagcat cccagcgcga cttacttcgt caaagcaagt ggtgattcta 3001 tgattgatgg tggaattagt gacggtgatt tactgattgt cgatagcgct attaccgcca 3061 gccatggtga tattgtcatc gctgctgttg acggcgagtt tacggtgaaa aaattgcaac 3121 tacgcccgac ggtacagctt attcccatga acagcgcgta ctcgcccatt accatcagta 3181 gtgaagatac gctggatgtc tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat 3241 gtttgccctc tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt ttcgccctga 3301 tttatggggt aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta tcgcccgaaa 3361 cgctgaggca aaggcgcttg gcgttaaaat gggcgatccc tggttcaaac aaaaagatct 3421 gtttcgtcgc tgtggcgtgg tttgctttag cagcaattat gagctttacg cagacatgag 3481 caatcgggtg atgtcgacgc tggaagagct atcgccccgc gtcgagattt acagtaccgg 3541 tatgcctatt gatgaggcat tctgcgatct gacaggtgtg cgtaattgtc gcgatctgac 3601 tgattttggc agagaaattc gcgcaacggt gctacaacgt acccatctta ctgttggtgt 3661 ggggatcgcc cagaccaaaa cgctggctaa gcttgccaat catgcggcaa aaaaatggca 3721 gcggcagacg ggtggggtgg tggatttatc aaatctggaa cgccagcgta aattaatgtc 3781 tgctctcccc gtggatgacg tctgggggat tggacggcgg atcagcaaaa aactggacgc 3841 gatggggatc aaaaccgttc tcgatttggc ggatacagat atccggttta tccgtaaaca 3901 ttttaatgtc gtgctcgaaa gaacggtgcg tgaactgcgc ggcgaaccct gtttgcaact 3961 ggaagagttt gcaccgacga agcaggaaat tatctgttcc cgctcgtttg gtgaacgcat 4021 cacggattat ccgtcgatgc ggcaggccat ttgtagttac gctgcccggg cggcggaaaa 4081 acttcgcagc gagcatcaat attgtcgatt aatttccacg tttattaaga cgtcaccatt 4141 tgcgctcaat gaaccttatt acggcaatag cgcgtcggta aaactgctga cgcccactca 4201 ggacagcagg gatatcatta acgctgctac gcgatctctg gatgccatct ggcaagcggg 4261 ccatcgttac caaaaagcgg gcgtgatgct gggggatttc ttcagtcagg gagtcgcgca 4321 gctcaattta ttcgatgaca acgcaccgcg ccccgggagt gagcaattga tgacggtaat 4381 ggatacactg aatgctaaag agggcagagg aacactctat tttgccgggc aggggatcca 4441 gcaacaatgg cagatgaagc gagccatgct ttcaccacgt tatacaacgc gaagttctga 4501 tttactgagg gtcaaataaa tatagcggca ggaaaaaagc gatcccgcat atccggtatt 4561 acccggcatg acaggagtaa aaatggctat cgacgaaaac aaacagaaag cgttggcggc 4621 agcactgggc cagattgaga aacaatttgg taaaggctcc atcatgcgcc tgggtgaaga 4681 ccgttccatg gatgtgaaaa ccatctctac cggttcgctt tcactggata tcgcgcttgg 4741 ggcaggtggt ctgccgatgg gccgtatcgt cgaaatctac ggaccggaat cttccggtaa 4801 aaccacgctg acgctgcagg tgatcgccgc agcgcagcgt gaaggtaaaa cctgtgcgtt 4861 tatcgatgct gaacacgcgc tggacccaat ctacgcacgt aaactgggcg tcgatatcga 4921 taacctgctg tgctcccagc cggacaccgg cgagcaggca ctggaaatct gtgacgccct 4981 ggcgcgttct ggcgcagtag acgttatcgt cgttgactcc gtggcggcac tgacgccgaa 5041 agcggaaatc gaaggcgaaa tcggcgactc tcacatgggc cttgcggcac gtatgatgag 5101 ccaggcgatg cgtaagctgg cgggtaacct gaagcagtcc aacacgctgc tgatcttcat 5161 caaccagatc cgtatgaaaa ttggtgtgat gttcggtaac ccggaaacca ctaccggtgg 5221 taacgcgctg aaattctacg cctctgttcg tctcgacatc cgtcgtatcg gcgcggtgaa 5281 agagggcgaa aacgtggtgg gtagcgaaac ccgcgtgaaa gtggtgaaga acaaaatcgc 5341 tgcgccgttt aaacaggctg aattccagat cctctacggc gaaggtatca acttctacgg 5401 cgaactggtt gacctgggcg taaaagagaa gctgatcgag aaagcaggcg cgtggtacag 5461 ctacaaaggt gagaagatcg gtcagggtaa agcgaatgcg actgcctggc tgaaagataa 5521 cccggaaacc gcgaaagaga tcgagaagaa agtacgtgag ttgctgctga gcaacccgaa 5581 ctcaacgccg gatttctctg tagatgatag cgaaggcgta gcagaaacta acgaagattt 5641 ttaaacagtc agtagggccc tggctgtttt ggcggatgag agaagatttt cagcctgata 5701 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc 5761 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt 5821 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc 5881 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag 5941 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg 6001 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga 6061 tggccttttt gcgtttctac aaactcgctg aggacattgg ctccgtgatg aaattcgcca 6121 cttgttagtg taattcgcta actcatcctg gcatgttgct gttgattctt caatcagatc 6181 tttataaatc aaaaagataa aaaattggca cgcaaattgt attaacagtt catccctatc 6241 agtgatagag agcaggacaa tcctgaacgc agaaatcaag aggacaacat tacctgcagg 6301 tgcagtaagg aggaaaaaaa aatgaaaaaa ttattattcg caattccttt agttgttcct 6361 ttctattctc actccgctga aactgttgaa agttgtttag caaaacccca tacagaaaat 6421 tcatttacta acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggc 6481 tgtctgtgga atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgttacggt 6541 acatgggttc ctattgggct tgctatccct gaaaatgagg gtggtggctc tgagggtggc 6601 ggttctgagg gtggcggttc tgagggtggc ggtactaaac ctcctgagta cggtgataca 6661 cctattccgg gctatactta tatcaaccct ctcgacggca cttatccgcc tggtactgag 6721 caaaaccccg ctaatcctaa tccttctctt gaggagtctc agcctcttaa tactttcatg 6781 tttcagaata ataggttccg aaataggcag ggggcattaa ctgtttatac gggcactgtt 6841 actcaaggca ctgaccccgt taaaacttat taccagtaca ctcctgtatc atcaaaagcc 6901 atgtatgacg cttactggaa cggtaaattc agagactgcg ctttccattc tggctttaat 6961 gaggatccat tcgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc 7021 aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg tggctctgag 7081 ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc 7141 ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc 7201 gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac 7261 ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct 7321 actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca 7381 cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc 7441 ccttttgtct ttggcgctgg taaaccttac gagttcagta tcgactgcga taagatcaac 7501 ctgttccgcg gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct 7561 acgtttgcta acatactgcg taataaggag tcttaaaggg tccctaagtc tcctcagcaa 7621 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgaccggat gtcctcttcc 7681 tcctgggctg acttcaggtg ctacatttga agagataaat tgcactgaaa tctagagcgg 7741 ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg 7801 ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg 7861 aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag 7921 tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc 7981 tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttaccgtgct tttccgggtt 8041 ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg 8101 cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta 8161 tgagaaagcg ccacacttcc cgtaagggag aaaggcggaa caggtatccg gtaaacggca 8221 gggtcggaac aggagagcgc aagagggagc gacccgccgg aaacggtggg gatctttaag 8281 tcctgtcggg tttcgcccgt actgtcagat tcatggttga gcctcacggc tcccacagat 8341 gcaccggaaa agcgtctgtt tatgtgaact ctggcaggag ggcggagcct atggaaaaac 8401 gccaccggcg cggccctgct gttttgcctc acatgttagt cccctgctta tccacggaat 8461 ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc 8521 gaccgagtg //