LOCUS BBa_J100271 8829 bp DNA linear 28-Mar-2024 DEFINITION SP-T7WT (Carlson et al. 2014) ACCESSION BBa_J100271 VERSION BBa_J100271.1 FEATURES Location/Qualifiers CDS 1..1233 /label=geneII CDS 898..1233 /label=geneX CDS 1245..1508 /label=geneV CDS 1510..1611 /label=geneVII CDS 1608..1706 /label=geneIX CDS 1703..1924 /label=geneVIII promoter 1910..1939 /label=geneIII promoter CDS 1987..2802 /label=KanR M13 SP CDS 2823..5474 /label=T7 RNAP SP WT CDS 5495..5677 /label=geneIII fragment CDS 5680..6018 /label=geneVI CDS 6020..7066 /label=geneI CDS 7044..8324 /label=geneIV misc_feature 8312..8767 /label=M13 Origin of Replication misc_feature 8329..8365 /label=F1 Origin of Replication promoter 8758..8786 /label=geneII promoter ORIGIN 1 atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg ctccagactc 61 tcaggcaatg acctgatagc ctttgtagac ctctcaaaaa tagctaccct ctccggcatg 121 aatttatcag ctagaacggt tgaatatcat gttgatggtg atttgactgt ctccggcctt 181 tctcaccctt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag 241 ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag 301 ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat 361 tttgctaatt ctttgccttg cctgtatgat ttattggatg ttaacgctac tactattagt 421 agaattgatg ccaccttttc agctcgcgcc ccaaatgaaa atatagctaa acaggttatt 481 gaccatttgc gaaatgtatc taatggtcaa actaaatcta ctcgttcgca gaattgggaa 541 tcaactgtta catggaatga aacttccaga caccgtactt tagttgcata tttaaaacat 601 gttgagctac agcaccagat tcagcaatta agctctaagc catccgcaaa aatgacctct 661 tatcaaaagg agcaattaaa ggtactctct aatcctgacc tgttggagtt tgcttccggg 721 ctggttcgct ttgaagctcg aattagaacg cgatatttga agtctttcgg gcttcctctt 781 aatctttttg atgcaatccg ctttgcttct gactataata gtcagggtaa agacctgatt 841 tttgatttat ggtcattctc gttttctgaa ctgtttaaag catttgaggg ggattcaatg 901 aatatttatg acgattccgc agtattggac gctatccagt ctaaacattt tactattacc 961 ccctctggca aaacttcttt tgcaaaagcc tctcgctatt ttggttttta tcgtcgtctg 1021 gtaaacgagg gttatgatag tgttgctctt actatgcctc gtaattcctt ttggcgttat 1081 gtatctgcat tagttgaatg tggtattcct aaatctcaac tgatgaatct ttctacctgt 1141 aataatgttg ttccgttagt tcgttttatt aacgtagatt tttcttccca acgtcctgac 1201 tggtataatg agccagttct taaaatcgca taaggtaatt cacaatgatt aaagttgaaa 1261 ttaaaccatc tcaagcccaa tttactactc gttctggtgt ttctcgtcag ggcaagcctt 1321 attcactgaa tgagcagctt tgttacgttg atttgggtaa tgaatatccg gttcttgtca 1381 agattactct tgatgaaggt cagccagcct atgcgcctgg tctgtacacc gttcatctgt 1441 cctctttcaa agttggtcag ttcggttccc ttatgattga ccgtctgcgc ctcgttccgg 1501 ctaagtaaca tggagcaggt cgcggatttc gacacaattt atcaggcgat gatacaaatc 1561 tccgttgtac tttgtttcgc gcttggtata atcgctgggg gtcaaagatg agtgttttag 1621 tgtattcttt cgcctctttc gttttaggtt ggtgccttcg tagtggcatt acgtatttta 1681 cccgtttaat ggaaacttcc tcatgaaaaa gtctttagtc ctcaaagcct ctgtagccgt 1741 tgctaccctc gttccgatgc tgtctttcgc tgctgagggt gacgatcccg caaaagcggc 1801 ctttaactcc ctgcaagcct cagcgaccga atatatcggt tatgcgtggg cgatggttgt 1861 tgtcattgtc ggcgcaacta tcggtatcaa gctgtttaag aaattcacct cgaaagcaag 1921 ctgataaacc gatacaatta aaggctcctt ttggagcctt ttttttggag taaggaggaa 1981 aaaagcatgc gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac 2041 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 2101 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 2161 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt 2221 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 2281 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 2341 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 2401 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 2461 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 2521 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat 2581 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 2641 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 2701 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 2761 aataaattgc agtttcattt gatgctcgat gagtttttct aacagctgag gaggaaaaaa 2821 aaatgaacac gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc 2881 cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg 2941 agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta 3001 aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta 3061 agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc 3121 cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta 3181 agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg 3241 caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta 3301 agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga 3361 aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg 3421 cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc 3481 tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag 3541 actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg 3601 cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg 3661 gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc 3721 acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga 3781 ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg 3841 taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac 3901 tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg 3961 ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt 4021 tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca 4081 tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga 4141 ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc 4201 tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct gagcgcatca 4261 agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca 4321 cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg 4381 gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt 4441 gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta 4501 acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg 4561 agattctaca agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg 4621 agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat 4681 ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg 4741 ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg 4801 attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc 4861 tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta 4921 agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc 4981 gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca 5041 agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc ttacagccta 5101 ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc 5161 ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg 5221 agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg 5281 acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg 5341 atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca 5401 aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact 5461 tcgcgttcgc gtaatctaga aggagatttt caacatgctc cctcaatcgg ttgaatgtcg 5521 cccttttgtc tttagcgctg gtaaaccata tgaattttct attgattgtg acaaaatgaa 5581 cttattccgt ggtgtctttg cgtttctttt atatgttgcc acctttatgt atgtattttc 5641 tacgtttgct aacatactgc gtaataagga gtcttaatca tgccagttct tttgggtatt 5701 ccgttattat tgcgtttcct cggtttcctt ctggtaactt tgttcggcta tctgcttact 5761 tttcttaaaa agggcttcgg taagatagct attgctattt cattgtttct tgctcttatt 5821 attgggctta actcaattct tgtgggttat ctctctgata ttagcgctca attaccctct 5881 gactttgttc agggtgttca gttaattctc ccgtctaatg cgcttccctg tttttatgtt 5941 attctctctg taaaggctgc tattttcatt tttgacgtta aacaaaaaat cgtttcttat 6001 ttggattggg ataaataata tggctgttta ttttgtaact ggcaaattag gctctggaaa 6061 gacgctcgtt agcgttggta agattcagga taaaattgta gctgggtgca aaatagcaac 6121 taatcttgat ttaaggcttc aaaacctccc gcaagtcggg aggttcgcta aaacgcctcg 6181 cgttcttaga ataccggata agccttctat atctgatttg cttgctattg ggcgcggtaa 6241 tgattcctac gatgaaaata aaaacggctt gcttgttctc gatgagtgcg gtacttggtt 6301 taatacccgt tcttggaatg ataaggaaag acagccgatt attgattggt ttctacatgc 6361 tcgtaaatta ggatgggata ttatttttct tgttcaggac ttatctattg ttgataaaca 6421 ggcgcgttct gcattagctg aacatgttgt ttattgtcgt cgtctggaca gaattacttt 6481 accttttgtc ggtactttat attctcttat tactggctcg aaaatgcctc tgcctaaatt 6541 acatgttggc gttgttaaat atggcgattc tcaattaagc cctactgttg agcgttggct 6601 ttatactggt aagaatttgt ataacgcata tgatactaaa caggcttttt ctagtaatta 6661 tgattccggt gtttattctt atttaacgcc ttatttatca cacggtcggt atttcaaacc 6721 attaaattta ggtcagaaga tgaaattaac taaaatatat ttgaaaaagt tttctcgcgt 6781 tctttgtctt gcgattggat ttgcatcagc atttacatat agttatataa cccaacctaa 6841 gccggaggtt aaaaaggtag tctctcagac ctatgatttt gataaattca ctattgactc 6901 ttctcagcgt cttaatctaa gctatcgcta tgttttcaag gattctaagg gaaaattaat 6961 taatagcgac gatttacaga agcaaggtta ttcactcaca tatattgatt tatgtactgt 7021 ttccattaaa aaaggtaatt caaatgaaat tgttaaatgt aattaatttt gttttcttga 7081 tgtttgtttc atcatcttct tttgctcagg taattgaaat gaataattcg cctctgcgcg 7141 attttgtaac ttggtattca aagcaatcag gcgaatccgt tattgtttct cccgatgtaa 7201 aaggtactgt tactgtatat tcatctgacg ttaaacctga aaatctacgc aatttcttta 7261 tttctgtttt acgtgcaagt aattttgata tggttggttc taacccttcc attattcaga 7321 agtataatcc aaacaatcag gattatattg atgaattgcc atcatctgat aatcaggaat 7381 atgatgataa ttccgctcct tctggtggtt tctttgttcc gcaaaatgat aatgttactc 7441 aaacttttaa aattaataac gttcgggcaa aggatttaat acgagttgtc gaattgtttg 7501 taaagtctaa tacttctaaa tcctcaaatg tattatctat tgacggctct aatctattag 7561 ttgttagtgc acctaaagat attttagata accttcctca attcctttct actgttgatt 7621 tgccaactga ccagatattg attgagggtt tgatatttga ggttcagcaa ggtgatgctt 7681 tagatttttc atttgctgct ggctctcagc gtggcactgt tgcaggcggt gttaatactg 7741 accgcctcac ctctgtttta tcttctgctg gtggttcgtt cggtattttt aatggcgatg 7801 ttttagggct atcagttcgc gcattaaaga ctaatagcca ttcaaaaata ttgtctgtgc 7861 cacgtattct tacgctttca ggtcagaagg gttctatctt tgttggccag aatgtccctt 7921 ttattactgg tcgtgtgact ggtgaatctg ccaatgtaaa taatccattt cagacgattg 7981 agcgtcaaaa tgtaggtatt tccatgagcg tttttcctgt tgcaatggct ggcggtaata 8041 ttgttctgga tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg 8101 ttattactaa tcaaagaagt actgctacaa cggttaattt gcgtgatgga cagactcttt 8161 tactcggtgg cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt 8221 ctaaaatccc tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca 8281 cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg 8341 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 8401 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 8461 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 8521 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 8581 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 8641 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg 8701 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 8761 acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctt attatcaacc 8821 ggggtacat //