LOCUS       BBa_J100272             1542 bp    DNA     linear       09-May-2024
DEFINITION  rClone Red Version 2: Device for GGA Cloning and Testing RBS 
            elements and Riboswitches
ACCESSION   BBa_J100272
VERSION     BBa_J100272.1  
FEATURES             Location/Qualifiers
     promoter        1..37
                     /label=P5 Promoter
     misc_feature    42..48
                     /label=BsaI Site
     CDS             55..775
                     /label=GFP
     RBS             781..793
                     /label=RBS B0034
     promoter        803..849
                     /label=P2 Promoter
     misc_feature    849..855
                     /label=BsaI Site
     CDS             862..1542
                     /label=Optimized E1010 RFP
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta
       61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
      361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
      541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta
      781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata
      841 ctctttttgg tctctgcgga gatggcgtca agtgaagatg ttatcaaaga gttcatgcgt
      901 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      961 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
     1021 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
     1081 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
     1141 gaacgtgtta tgaacttcga agatggtggt gttgttaccg ttacccagga ctcctccctg
     1201 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
     1261 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
     1321 gatggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
     1381 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
     1441 aaaaccgaca tcaaactgga catcacctcc cacaacgaag attacaccat cgttgaacag
     1501 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aa
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