BBa_J100274 1 BBa_J100274 tCloneTet+PDB riboswitch with P14 2016-06-23T11:00:00Z 2016-06-24T09:27:28Z J100255 The tCloneTet+PDB plasmid was obtained from part J100255 and was modified using Golden Gate Assembly to have a P14 promoter rather than the P5 promoter. The P14 promoter is known to have a lower efficiency than the P5 and the P2 promoter but has a higher efficiency than the P9 and P4 promoters. false false _578_ 23967 23967 9 false Used PCR to "cut out" the P5 promoter since there were no restriction enzyme sites surrounding it. false Anna Buser annotation2480757 1 PDB riboswitch range2480757 1 41 112 annotation2480760 1 TetA range2480760 1 202 1398 annotation2480756 1 P14 range2480756 1 1 36 annotation2480758 1 BD18 C Dog RBS range2480758 1 117 201 BBa_J100274_sequence 1 ttgacaattaatcatccggctcgtataatgtgtggacgacggcgataccagccgaaaggcccttggcagcgtctttacatactcggtaaacgacgctgccaaggttttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgggacttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z