BBa_J100277 1 BBa_J100277 tCloneTet+PDB riboswitch with P14 and BD10 2016-06-23T11:00:00Z 2016-06-24T10:14:45Z J100274 The BD10 C Dog Ribosome Binding Site has a lower binding efficiency than BD18. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100274 and insert in the BD10 C Dog RBS. false false _578_ 23967 23967 9 false Since there was only a 6 base difference between BD18 C Dog and BD10 C Dog, the PCR was used to insert in and amplify the new BD10 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid. false Anna Buser annotation2480776 1 P14 range2480776 1 1 36 annotation2480777 1 PDB riboswitch range2480777 1 41 112 annotation2480779 1 TetA range2480779 1 202 1398 annotation2480778 1 BD10 C Dog RBS range2480778 1 117 201 BBa_J100277_sequence 1 ttgacaattaatcatccggctcgtataatgtgtggacgacggcgataccagccgaaaggcccttggcagcgtctttacatactcggtaaacgacgctgccaaggttttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcggaggatcgtttctaatgggacttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z