LOCUS       BBa_J100281             2797 bp    DNA     linear       23-Dec-2024
DEFINITION  tetA+sacB without RBS
ACCESSION   BBa_J100281
VERSION     BBa_J100281.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=pBAD
     CDS             137..1324
                     /label=tetA
     misc_feature    1325..1372
                     /label=linker
     CDS             1373..2797
                     /label=sacB
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
      181 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
      241 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
      301 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
      361 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
      421 cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      481 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      541 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      601 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      661 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag
      721 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
      781 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
      841 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
      901 cggaatcttg cacgccctcg ctcaagcctt cgtcactggc cccgccacca aacgtttcgg
      961 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
     1021 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     1081 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     1141 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
     1201 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     1261 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     1321 gaccggtggt ggaagtggtg gtggttcagg tggtggttct ggaggcggtt ctatgaacat
     1381 caaaaaattc gcaaaacagg caaccgttct gacctttacc accgcactgc tggcaggcgg
     1441 tgcaacccag gcatttgcaa aagaaaccaa tcagaaaccg tataaagaaa cgtatggcat
     1501 cagccatatt acccgtcatg atatgctgca aattccggaa cagcagaaaa acgaaaaata
     1561 tcaggtgccc gaatttgata gcagcacgat taaaaacatt agcagcgcaa aaggtctgga
     1621 tgtttgggat agctggccgt tacagaatgc agatggcacc gttgcaaatt atcatggcta
     1681 tcacattgtt tttgccttag ccggtgatcc gaaaaatgcc gatgatacca gcatctatat
     1741 gttttatcag aaagtgggcg aaaccagcat tgacagctgg aaaaacgcag gtcgtgtgtt
     1801 taaagatagc gataaatttg atgccaacga tagcatcctg aaagatcaga cccaagaatg
     1861 gtcaggtagc gcaaccttta caagtgatgg taaaatccgt ctgttctata ccgatttcag
     1921 cggtaaacat tatggtaaac agaccctgac cacagcacag gttaatgtta gcgcaagcga
     1981 tagcagtctg aatattaacg gtgtggaaga ttacaaaagc atctttgatg gtgatggcaa
     2041 aacctaccag aatgtgcagc agtttattga tgaaggcaat tatagcagcg gtgataatca
     2101 taccctgcgt gatccgcatt atgttgaaga taaaggccat aaatatctgg tgtttgaagc
     2161 aaataccggc accgaagatg gttatcaggg tgaagaaagc ctgtttaaca aagcctatta
     2221 tggcaaatcg accagctttt ttcgtcaaga aagtcagaaa ctgttacaga gcgacaaaaa
     2281 acgtaccgca gaactggcaa atggtgcact gggcatgatt gaactgaatg atgattacac
     2341 cctgaaaaaa gtgatgaaac cgctgattgc aagcaatacc gtgaccgatg aaattgaacg
     2401 tgccaatgtg ttcaaaatga acggcaagtg gtatttattc accgatagcc gtggtagcaa
     2461 aatgaccatt gatggtatta cgagcaacga catttatatg ctgggttatg tgagcaatag
     2521 tctgaccggt ccttataaac ctctgaataa aaccggtctg gtgctgaaaa tggatctgga
     2581 tccgaatgat gtgacattta cctatagcca ttttgcagtt ccgcaggcca aaggtaataa
     2641 tgttgttatt accagctaca tgaccaaccg tggtttttat gcagataaac agagcacctt
     2701 tgcaccgagc tttttactga acattaaagg caagaaaacc agcgttgtga aagacagcat
     2761 tctggaacag ggacagctga ccgtgaataa gtaataa
//