BBa_J100281 1 BBa_J100281 tetA+sacB without RBS 2016-06-24T11:00:00Z 2016-07-08T12:52:04Z pBAD-RBS-tetA from Part K091133 sacB from Part J119042 linker sequence optimized from Part J119386 Fusion protein of tetA and sacB connected with a linker sequence, with a pBAD promotor and RBS. TetA encodes tetracycline resistance and sacB kills E. coli in the presence of sucrose, allowing for both positive and negative selection. pBAD is an arabinose-inducible promoter. false false _578_ 28812 28812 9 false Linker sequence was modified to optimize the GC content of the primers. false Hartlee Johnston annotation2480939 1 linker range2480939 1 1325 1372 annotation2480940 1 sacB range2480940 1 1373 2797 annotation2480938 1 tetA range2480938 1 137 1324 annotation2480937 1 pBAD range2480937 1 1 130 BBa_J100281_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgaccggtggtggaagtggtggtggttcaggtggtggttctggaggcggttctatgaacatcaaaaaattcgcaaaacaggcaaccgttctgacctttaccaccgcactgctggcaggcggtgcaacccaggcatttgcaaaagaaaccaatcagaaaccgtataaagaaacgtatggcatcagccatattacccgtcatgatatgctgcaaattccggaacagcagaaaaacgaaaaatatcaggtgcccgaatttgatagcagcacgattaaaaacattagcagcgcaaaaggtctggatgtttgggatagctggccgttacagaatgcagatggcaccgttgcaaattatcatggctatcacattgtttttgccttagccggtgatccgaaaaatgccgatgataccagcatctatatgttttatcagaaagtgggcgaaaccagcattgacagctggaaaaacgcaggtcgtgtgtttaaagatagcgataaatttgatgccaacgatagcatcctgaaagatcagacccaagaatggtcaggtagcgcaacctttacaagtgatggtaaaatccgtctgttctataccgatttcagcggtaaacattatggtaaacagaccctgaccacagcacaggttaatgttagcgcaagcgatagcagtctgaatattaacggtgtggaagattacaaaagcatctttgatggtgatggcaaaacctaccagaatgtgcagcagtttattgatgaaggcaattatagcagcggtgataatcataccctgcgtgatccgcattatgttgaagataaaggccataaatatctggtgtttgaagcaaataccggcaccgaagatggttatcagggtgaagaaagcctgtttaacaaagcctattatggcaaatcgaccagcttttttcgtcaagaaagtcagaaactgttacagagcgacaaaaaacgtaccgcagaactggcaaatggtgcactgggcatgattgaactgaatgatgattacaccctgaaaaaagtgatgaaaccgctgattgcaagcaataccgtgaccgatgaaattgaacgtgccaatgtgttcaaaatgaacggcaagtggtatttattcaccgatagccgtggtagcaaaatgaccattgatggtattacgagcaacgacatttatatgctgggttatgtgagcaatagtctgaccggtccttataaacctctgaataaaaccggtctggtgctgaaaatggatctggatccgaatgatgtgacatttacctatagccattttgcagttccgcaggccaaaggtaataatgttgttattaccagctacatgaccaaccgtggtttttatgcagataaacagagcacctttgcaccgagctttttactgaacattaaaggcaagaaaaccagcgttgtgaaagacagcattctggaacagggacagctgaccgtgaataagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z