LOCUS BBa_J100284 3760 bp DNA linear 26-Dec-2024 DEFINITION JC184d5 with Mutagenesis Cassette Removed ACCESSION BBa_J100284 VERSION BBa_J100284.1 FEATURES Location/Qualifiers promoter 17..114 /label=cat CmR Promoter CDS 115..774 /label=cat CmR CDS 874..875 /label=recA730 misc_feature 893..1317 /label=rrnB1 Transcriptional Terminator misc_feature 1334..1463 /label=psp promoter 1464..1482 /label=tetR binding site (175h) RBS 1538..1552 /label=SD8 RBS CDS 1553..2827 /label=geneIII misc_feature 2984..3722 /label=CloDF13 Origin of Replication misc_feature 2850..2894 /label=rrnB1 Transcriptional Terminator ORIGIN 1 acggcctatt tggcctggca cgtaagaggt tccaactttc accataatga aataagatca 61 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag 121 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 181 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 241 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 301 cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg 361 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 421 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 481 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 541 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 601 atggacaact tcttcgcccc cgttttcact atgggcaaat attatacgca aggcgacaag 661 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc 721 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcca 781 tgggcattaa aaaatgccct cttgggttat caagagggtc attatatttc gcaaaaaacc 841 ccgcttcggc ggggtttttt cgcaggcggc cgcaaacagt cagtagggcc ctggctgttt 901 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct 961 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa 1021 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg 1081 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1141 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga 1201 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc 1261 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcgct 1321 gaggacattg gctccgtgat gaaattcgcc acttgttagt gtaattcgct aactcatcct 1381 ggcatgttgc tgttgattct tcaatcagat ctttataaat caaaaagata aaaaattggc 1441 acgcaaattg tattaacagt tcatccctat cagtgataga gagcaggaca atcctgaacg 1501 cagaaatcaa gaggacaaca ttacctgcag gtgcagtaag gaggaaaaaa aaatgaaaaa 1561 attattattc gcaattcctt tagttgttcc tttctattct cactccgctg aaactgttga 1621 aagttgttta gcaaaacccc atacagaaaa ttcatttact aacgtctgga aagacgacaa 1681 aactttagat cgttacgcta actatgaggg ctgtctgtgg aatgctacag gcgttgtagt 1741 ttgtactggt gacgaaactc agtgttacgg tacatgggtt cctattgggc ttgctatccc 1801 tgaaaatgag ggtggtggct ctgagggtgg cggttctgag ggtggcggtt ctgagggtgg 1861 cggtactaaa cctcctgagt acggtgatac acctattccg ggctatactt atatcaaccc 1921 tctcgacggc acttatccgc ctggtactga gcaaaacccc gctaatccta atccttctct 1981 tgaggagtct cagcctctta atactttcat gtttcagaat aataggttcc gaaataggca 2041 gggggcatta actgtttata cgggcactgt tactcaaggc actgaccccg ttaaaactta 2101 ttaccagtac actcctgtat catcaaaagc catgtatgac gcttactgga acggtaaatt 2161 cagagactgc gctttccatt ctggctttaa tgaggatcca ttcgtttgtg aatatcaagg 2221 ccaatcgtct gacctgcctc aacctcctgt caatgctggc ggcggctctg gtggtggttc 2281 tggtggcggc tctgagggtg gtggctctga gggtggcggt tctgagggtg gcggctctga 2341 gggaggcggt tccggtggtg gctctggttc cggtgatttt gattatgaaa agatggcaaa 2401 cgctaataag ggggctatga ccgaaaatgc cgatgaaaac gcgctacagt ctgacgctaa 2461 aggcaaactt gattctgtcg ctactgatta cggtgctgct atcgatggtt tcattggtga 2521 cgtttccggc cttgctaatg gtaatggtgc tactggtgat tttgctggct ctaattccca 2581 aatggctcaa gtcggtgacg gtgataattc acctttaatg aataatttcc gtcaatattt 2641 accttccctc cctcaatcgg ttgaatgtcg cccttttgtc tttggcgctg gtaaacctta 2701 cgagttcagt atcgactgcg ataagatcaa cctgttccgc ggtgtctttg cgtttctttt 2761 atatgttgcc acctttatgt atgtattttc tacgtttgct aacatactgc gtaataagga 2821 gtcttaaagg gtccctaagt ctcctcagca aaacgaaagg cccagtcttt cgactgagcc 2881 tttcgtttta tttgaccgga tgtcctcttc ctcctgggct gacttcaggt gctacatttg 2941 aagagataaa ttgcactgaa atctagagcg gttcagtaga aaagatcaaa ggatcttctt 3001 gagatccttt ttttctgcgc gtaatctttt gccctgtaaa cgaaaaaacc acctggggag 3061 gtggtttgat cgaaggttaa gtcagttggg gaactgctta accgtggtaa ctggctttcg 3121 cagagcacag caaccaaatc tgtccttcca gtgtagccgg actttggcgc acacttcaag 3181 agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc ccagttacca atggctgctg 3241 ccagtggcgt tttaccgtgc ttttccgggt tggactcaag tgaacagtta ccggataagg 3301 cgcagcagtc gggctgaacg gggagttctt gcttacagcc cagcttggag cgaacgacct 3361 acaccgagcc gagataccag tgtgtgagct atgagaaagc gccacacttc ccgtaaggga 3421 gaaaggcgga acaggtatcc ggtaaacggc agggtcggaa caggagagcg caagagggag 3481 cgacccgccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga 3541 ttcatggttg agcctcacgg ctcccacaga tgcaccggaa aagcgtctgt ttatgtgaac 3601 tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc tgttttgcct 3661 cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg tgtccgcagc 3721 gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg //