BBa_J100302
1
BBa_J100302
asr promoter (trimmed version of K1231000)
2016-09-07T11:00:00Z
2016-09-08T01:38:54Z
Registry of Standard Biological Parts: Part:BBa_K1231000
This asr promoter induces transcription at acidic conditions, approximately at a pH of 5.5. It is native to E. coli.
false
false
_578_
33877
33877
9
false
There was no recorded -10 or -35 sequence. We identified possible -10 sequences and used a position weight matrix to determine the most likely -10 sequence. Then we determined the -35 position by counting to the left 25 nucleotides. We cut the promoter to 56 bases that contained both consensus sequences. We inputed that sequence into the Oligator which added on the sticky ends according to the Golden Gate Assembly method.
false
Jackson Miller
annotation2482707
1
-35 box
range2482707
1
20
26
annotation2482706
1
-10 box
range2482706
1
45
51
BBa_J100302_sequence
1
ttatttcaacggccccgcagtggggttaaatgaaaaaacaaattgagggtatgaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z