BBa_J100302 1 BBa_J100302 asr promoter (trimmed version of K1231000) 2016-09-07T11:00:00Z 2016-09-08T01:38:54Z Registry of Standard Biological Parts: Part:BBa_K1231000 This asr promoter induces transcription at acidic conditions, approximately at a pH of 5.5. It is native to E. coli. false false _578_ 33877 33877 9 false There was no recorded -10 or -35 sequence. We identified possible -10 sequences and used a position weight matrix to determine the most likely -10 sequence. Then we determined the -35 position by counting to the left 25 nucleotides. We cut the promoter to 56 bases that contained both consensus sequences. We inputed that sequence into the Oligator which added on the sticky ends according to the Golden Gate Assembly method. false Jackson Miller annotation2482707 1 -35 box range2482707 1 20 26 annotation2482706 1 -10 box range2482706 1 45 51 BBa_J100302_sequence 1 ttatttcaacggccccgcagtggggttaaatgaaaaaacaaattgagggtatgaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z