BBa_J100306 1 BBa_J100306 repClone Red (J100205) with wt TetR promoter (R0040) 2016-10-07T11:00:00Z 2016-10-10T11:48:04Z This part can be constructed by ligating the TetR promoter into repClone (J100205) following BsaI digestion. repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system. false false _578_ 18858 18858 9 false None. false Monica Prudencio annotation2488177 1 tetR Repressor C0041 range2488177 1 1 639 annotation2488180 1 GFP Optimized Sequence range2488180 1 764 1483 annotation2488181 1 B0034 range2488181 1 1490 1501 annotation2488183 1 TetR Repressible Promoter (B0040) range2488183 1 1516 1569 annotation2488184 1 BsaI Sticky End range2488184 1 1570 1573 annotation2488182 1 BsaI Sticky End range2488182 1 1512 1515 annotation2488185 1 BD18 range2488185 1 1574 1661 annotation2488179 1 P5 Promoter (for tetR) range2488179 1 728 763 annotation2488186 1 RFP range2488186 1 1659 2339 annotation2488178 1 BD24 range2488178 1 640 727 BBa_J100306_sequence 1 cggccgctactagtattattagctaccgctttcacatttcagttgtttttccagtccgcaaataatcagttctaaaccaaacagaaaggcaggttctgcaccctgatgatcaaacagttcaatggcctgacgcagcagcggaggcatgctatcggtggtcggggtttcgcgttcttcttttgcaacctgatgttcttgatcttccagaacacaacccagggtaaaatgaccaactgcgctcagtgcatacagtgcgttttccagactaaaaccttgctgacacagaaatgccagctgattttccagggtttcatactgtttttcggtcggacgggtgcccagatgaacttttgcaccatcacgatggctcagcagggcacaacgaaagctttttgcattattacgcagaaaatcctgccagctttcaccttccagcggacaaaaatgggtatgatgacgatccagcatttcaattgccagtgcatccagcagtgcacgtttgtttttaacatgccaatacagggtcggctgttcaacacccagtttctgtgccagtttacgggtggtcagaccttcaataccaacttcattcagcagttccagtgcgctattaatcactttgcttttatccagacggctcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccctccacataaattacgagccggatgattaattgtcaattattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctctcttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatatttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatacagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcgccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcacctagtatttctcctctttaattactagacgactccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z