LOCUS       BBa_J100308             2339 bp    DNA     linear       22-Dec-2024
DEFINITION  Variant of repClone Red(J100205) w/ wt TetR promoter (R0040)
ACCESSION   BBa_J100308
VERSION     BBa_J100308.1  
FEATURES             Location/Qualifiers
     CDS             1..639
                     /label=tetR Repressor C0041
     misc_feature    243..243
                     /label=G->T
     RBS             640..727
                     /label=BD24
     promoter        728..763
                     /label=P5 Promoter
     CDS             764..1483
                     /label=GFP Optimized
     misc_feature    945..945
                     /label=T->C
     misc_feature    953..953
                     /label=T->C
     RBS             1490..1501
                     /label=B0034
     misc_feature    1512..1515
                     /label=BsaI Sticky End
     promoter        1516..1569
                     /label=TetR Repressible Promoter
     misc_feature    1570..1573
                     /label=BsaI Sticky End
     RBS             1574..1661
                     /label=BD18
     CDS             1659..2339
                     /label=RFP
ORIGIN
        1 cggccgctac tagtattatt agctaccgct ttcacatttc agttgttttt ccagtccgca
       61 aataatcagt tctaaaccaa acagaaaggc aggttctgca ccctgatgat caaacagttc
      121 aatggcctga cgcagcagcg gaggcatgct atcggtggtc ggggtttcgc gttcttcttt
      181 tgcaacctga tgttcttgat cttccagaac acaacccagg gtaaaatgac caactgcgct
      241 cattgcatac agtgcgtttt ccagactaaa accttgctga cacagaaatg ccagctgatt
      301 ttccagggtt tcatactgtt tttcggtcgg acgggtgccc agatgaactt ttgcaccatc
      361 acgatggctc agcagggcac aacgaaagct ttttgcatta ttacgcagaa aatcctgcca
      421 gctttcacct tccagcggac aaaaatgggt atgatgacga tccagcattt caattgccag
      481 tgcatccagc agtgcacgtt tgtttttaac atgccaatac agggtcggct gttcaacacc
      541 cagtttctgt gccagtttac gggtggtcag accttcaata ccaacttcat tcagcagttc
      601 cagtgcgcta ttaatcactt tgcttttatc cagacggctc attagaaacc gtccatcgca
      661 tgattaagat gtttcagtac gaaaattgct ttcattgttg atctcctttt taagtgaact
      721 tgggccctcc acataaatta cgagccggat gattaattgt caattattat ttgtatagtt
      781 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtgatctc
      841 tctcttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      901 aaaggacagg gccatcgcca attggagtat tttgttgata atggcctgct agctgaacgc
      961 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
     1021 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatat
     1081 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
     1141 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat acagttcttt
     1201 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
     1261 ggtatctcgc aaagcattga acaccataac cgaaagtagt gacaagtgtt ggccatggaa
     1321 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     1381 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcgccatct aattcaacaa
     1441 gaattgggac aactccagtg aaaagttctt ctcctttacg cacctagtat ttctcctctt
     1501 taattactag acgactccct atcagtgata gagattgaca tccctatcag tgatagagat
     1561 actgagcacg cgggggccca agttcactta aaaaggagat caacaatgaa agcaattttc
     1621 gtactgaaac atcttaatca tgcgacggag cgtttctaat ggcttcctcc gaagatgtta
     1681 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
     1741 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
     1801 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     1861 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     1921 cggaaggttt caaatgggaa cgtgttatga acttcgaaga tggtggtgtt gttaccgtta
     1981 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     2041 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     2101 aacgtatgta cccggaagat ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     2161 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     2221 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagatt
     2281 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataa
//