##gff-version 3
##sequence-region BBa_J100308 1 2339
BBa_J100308	.	ribosome_entry_site	1574	1661	.	+	0	ID=annotation2491220;Name=BD18
BBa_J100308	.	sequence_alteration	945	945	.	+	0	ID=annotation2491214;Name=T->C
BBa_J100308	.	sequence_alteration	953	953	.	+	0	ID=annotation2491215;Name=T->C
BBa_J100308	.	ribosome_entry_site	1490	1501	.	+	0	ID=annotation2491216;Name=B0034
BBa_J100308	.	CDS	1659	2339	.	+	0	ID=annotation2491221;Name=RFP
BBa_J100308	.	promoter	1516	1569	.	+	0	ID=annotation2491218;Name=TetR Repressible Promoter
BBa_J100308	.	CDS	1	639	.	+	0	ID=annotation2491209;Name=tetR Repressor C0041
BBa_J100308	.	sequence_feature	1512	1515	.	+	0	ID=annotation2491217;Name=BsaI Sticky End
BBa_J100308	.	CDS	764	1483	.	+	0	ID=annotation2491213;Name=GFP Optimized
BBa_J100308	.	sequence_feature	1570	1573	.	+	0	ID=annotation2491219;Name=BsaI Sticky End
BBa_J100308	.	promoter	728	763	.	+	0	ID=annotation2491212;Name=P5 Promoter
BBa_J100308	.	sequence_alteration	243	243	.	+	0	ID=annotation2491210;Name=G->T
BBa_J100308	.	ribosome_entry_site	640	727	.	+	0	ID=annotation2491211;Name=BD24
>BBa_J100308
cggccgctactagtattattagctaccgctttcacatttcagttgtttttccagtccgcaaataatcagttctaaacca
aacagaaaggcaggttctgcaccctgatgatcaaacagttcaatggcctgacgcagcagcggaggcatgctatcggtgg
tcggggtttcgcgttcttcttttgcaacctgatgttcttgatcttccagaacacaacccagggtaaaatgaccaactgc
gctcattgcatacagtgcgttttccagactaaaaccttgctgacacagaaatgccagctgattttccagggtttcatac
tgtttttcggtcggacgggtgcccagatgaacttttgcaccatcacgatggctcagcagggcacaacgaaagctttttg
cattattacgcagaaaatcctgccagctttcaccttccagcggacaaaaatgggtatgatgacgatccagcatttcaat
tgccagtgcatccagcagtgcacgtttgtttttaacatgccaatacagggtcggctgttcaacacccagtttctgtgcc
agtttacgggtggtcagaccttcaataccaacttcattcagcagttccagtgcgctattaatcactttgcttttatcca
gacggctcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctccttttt
aagtgaacttgggccctccacataaattacgagccggatgattaattgtcaattattatttgtatagttcatccatgcc
atgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctctcttcgttgggatctttcgaaagggca
gattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggcctg
ctagctgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccat
gatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatatttccatcttctttaaaatcaatacct
tttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttga
aaaatacagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggta
tctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaa
ataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacat
cgccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcacctagtatttctcctcttt
aattactagacgactccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgcgggggccca
agttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaa
tggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa