BBa_J100308
1
BBa_J100308
Variant of repClone Red(J100205) w/ wt TetR promoter (R0040)
2016-10-09T11:00:00Z
2016-10-10T01:33:06Z
Insertion of R0040 promoter in J100307 (variant repClone).
repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system. This will allow researchers and biology students to explore sequence requirements for the interactions between the TetR repressible promoter, TetR protein, and aTc.
false
false
_578_
18858
18858
9
false
This part was constructed using Golden Gate Assembly to ligate the TetR promoter into variant repClone (J100307) following BsaI digestion.
false
Monica Prudencio
annotation2491220
1
BD18
range2491220
1
1574
1661
annotation2491216
1
B0034
range2491216
1
1490
1501
annotation2491221
1
RFP
range2491221
1
1659
2339
annotation2491217
1
BsaI Sticky End
range2491217
1
1512
1515
annotation2491209
1
tetR Repressor C0041
range2491209
1
1
639
annotation2491218
1
TetR Repressible Promoter
range2491218
1
1516
1569
annotation2491210
1
G->T
range2491210
1
243
243
annotation2491213
1
GFP Optimized
range2491213
1
764
1483
annotation2491212
1
P5 Promoter
range2491212
1
728
763
annotation2491214
1
T->C
range2491214
1
945
945
annotation2491219
1
BsaI Sticky End
range2491219
1
1570
1573
annotation2491215
1
T->C
range2491215
1
953
953
annotation2491211
1
BD24
range2491211
1
640
727
BBa_J100308_sequence
1
cggccgctactagtattattagctaccgctttcacatttcagttgtttttccagtccgcaaataatcagttctaaaccaaacagaaaggcaggttctgcaccctgatgatcaaacagttcaatggcctgacgcagcagcggaggcatgctatcggtggtcggggtttcgcgttcttcttttgcaacctgatgttcttgatcttccagaacacaacccagggtaaaatgaccaactgcgctcattgcatacagtgcgttttccagactaaaaccttgctgacacagaaatgccagctgattttccagggtttcatactgtttttcggtcggacgggtgcccagatgaacttttgcaccatcacgatggctcagcagggcacaacgaaagctttttgcattattacgcagaaaatcctgccagctttcaccttccagcggacaaaaatgggtatgatgacgatccagcatttcaattgccagtgcatccagcagtgcacgtttgtttttaacatgccaatacagggtcggctgttcaacacccagtttctgtgccagtttacgggtggtcagaccttcaataccaacttcattcagcagttccagtgcgctattaatcactttgcttttatccagacggctcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccctccacataaattacgagccggatgattaattgtcaattattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctctcttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggcctgctagctgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatatttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatacagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcgccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcacctagtatttctcctctttaattactagacgactccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z