LOCUS       BBa_J100309             1683 bp    DNA     linear       23-Dec-2024
DEFINITION  actClone Red with wt full OmpR promoter
ACCESSION   BBa_J100309
VERSION     BBa_J100309.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP
     RBS             721..805
                     /label=BD24
     misc_feature    806..809
                     /label=BsaI sticky end
     promoter        810..917
                     /label=OmpR (R0082)
     RBS             918..1002
                     /label=BD21
     CDS             1003..1683
                     /label=RFP (codon optimized)
ORIGIN
        1 tcatcatttg tacagttcat ccataccatg cgtgatgccc gctgcggtta cgaactccag
       61 cagaaccata tgatcgcgtt tctcgttcgg atctttagac agaacgcttt gcgtgctcag
      121 atagtgattg tctggcagca gaacaggacc atcaccgatt ggagtgtttt gctggtagtg
      181 atcagccagc tgcacgctgc catcctccac gttgtggcga attttaaaat tcgctttaat
      241 gccatttttt tgtttatcgg cggtgatgta aacattgtgg ctgttaaaat tgtattccag
      301 cttatggccc aggatattgc cgtcttcttt aaagtcaatg cctttcagct caatgcggtt
      361 taccagggta tcgccttcaa atttcacttc cgcacgcgtt ttgtacgtgc cgtcatcctt
      421 aaaggaaatc gtgcgttcct gcacatagcc ttccggcatg gcggacttga agaagtcatg
      481 ctgcttcata tggtccggat aacgagcaaa gcactgaaca ccataagtca gcgtcgttac
      541 cagagtcggc caaggtaccg gcagtttacc agtagtacag atgaacttca gcgtcagttt
      601 accattagtt gcgtcacctt caccctcgcc acgcacggaa aacttatgac cgttgacatc
      661 accatccagt tccaccagaa tagggacgac accagtgaac agctcttcgc ctttacgcat
      721 tagaaaccgt ccatcgcatg attaagatgt ttcagtacga aaattgcttt cattgttgat
      781 ctccttttta agtgaacttg ggccccgact cccttgcatt tacattttga aacatctata
      841 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt
      901 tttggggaga atggactggg cccaagttca cttaaaaagg agatcaacaa tgaaagcaat
      961 tttcgtactg aaacatctta atcatgcgag ggatggtttc taatggcaag cagcgaagat
     1021 gtgattaaag aatttatgcg cttcaaggtg cgtatggaag gtagcgttaa tggtcatgaa
     1081 tttgaaattg aaggtgaagg cgaaggtcgt ccgtatgaag gcacccagac cgcaaaactg
     1141 aaagttacca aaggtggtcc gctgccgttt gcatgggata ttctgagtcc gcagtttcag
     1201 tatggtagca aagcatatgt taaacatccg gcagatattc cggattacct gaaattaagc
     1261 tttccggaag gttttaaatg ggaacgcgtg atgaattttg aagatggtgg tgttgttacc
     1321 gttacccagg atagcagctt acaggatggt gagtttatct acaaagttaa actgcgtggc
     1381 accaactttc cgagtgatgg tccggttatg cagaaaaaaa ccatgggttg ggaagcaagc
     1441 accgaacgta tgtatccgga agatggcgca ctgaaaggtg aaatcaaaat gcgtctgaag
     1501 ctgaaagacg gtggtcatta tgatgcagaa gttaaaacca cctacatggc caaaaaaccg
     1561 gttcagctgc ctggtgcata taaaaccgat attaaactgg atatcaccag ccacaacgag
     1621 gattatacca ttgttgaaca gtatgaacgt gcagaaggtc gccatagtac cggtgcctaa
     1681 taa
//