BBa_J100309
1
BBa_J100309
actClone Red with wt full OmpR promoter
2016-10-27T11:00:00Z
2016-10-28T03:52:41Z
This part can be constructed by ligating the first 70 bp of the 5' end of the OmpR promoter (R0082) promoter into actClone (J100204) following BsaI digestion.
actClone Red is a construct we designed to allow students and researchers to investigate the OmpR promoter. The RFP reporter system in actClone provides phenotypic evidence of the action of the OmpR promoter for the transcription of RFP. This construct uses the wild type OmpR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs to RFP expression using the wild-type promoter sequence (R0082). This will allow researchers and biology students to explore sequence requirements for the interactions between the OmpR promoter and phosphorylated OmpR regulatory protein.
false
false
_578_
18858
18858
9
false
None
false
Monica Prudencio
annotation2530714
1
GFP
range2530714
1
1
720
annotation2530716
1
BsaI sticky end
range2530716
1
806
809
annotation2530719
1
RFP (codon optimized)
range2530719
1
1003
1683
annotation2530717
1
OmpR (R0082)
range2530717
1
810
917
annotation2530715
1
BD24
range2530715
1
721
805
annotation2530718
1
BD21
range2530718
1
918
1002
BBa_J100309_sequence
1
tcatcatttgtacagttcatccataccatgcgtgatgcccgctgcggttacgaactccagcagaaccatatgatcgcgtttctcgttcggatctttagacagaacgctttgcgtgctcagatagtgattgtctggcagcagaacaggaccatcaccgattggagtgttttgctggtagtgatcagccagctgcacgctgccatcctccacgttgtggcgaattttaaaattcgctttaatgccatttttttgtttatcggcggtgatgtaaacattgtggctgttaaaattgtattccagcttatggcccaggatattgccgtcttctttaaagtcaatgcctttcagctcaatgcggtttaccagggtatcgccttcaaatttcacttccgcacgcgttttgtacgtgccgtcatccttaaaggaaatcgtgcgttcctgcacatagccttccggcatggcggacttgaagaagtcatgctgcttcatatggtccggataacgagcaaagcactgaacaccataagtcagcgtcgttaccagagtcggccaaggtaccggcagtttaccagtagtacagatgaacttcagcgtcagtttaccattagttgcgtcaccttcaccctcgccacgcacggaaaacttatgaccgttgacatcaccatccagttccaccagaatagggacgacaccagtgaacagctcttcgcctttacgcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccccgactcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgagggatggtttctaatggcaagcagcgaagatgtgattaaagaatttatgcgcttcaaggtgcgtatggaaggtagcgttaatggtcatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatattccggattacctgaaattaagctttccggaaggttttaaatgggaacgcgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcttacaggatggtgagtttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgtctgaagctgaaagacggtggtcattatgatgcagaagttaaaaccacctacatggccaaaaaaccggttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagtaccggtgcctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z