BBa_J100309 1 BBa_J100309 actClone Red with wt full OmpR promoter 2016-10-27T11:00:00Z 2016-10-28T03:52:41Z This part can be constructed by ligating the first 70 bp of the 5' end of the OmpR promoter (R0082) promoter into actClone (J100204) following BsaI digestion. actClone Red is a construct we designed to allow students and researchers to investigate the OmpR promoter. The RFP reporter system in actClone provides phenotypic evidence of the action of the OmpR promoter for the transcription of RFP. This construct uses the wild type OmpR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs to RFP expression using the wild-type promoter sequence (R0082). This will allow researchers and biology students to explore sequence requirements for the interactions between the OmpR promoter and phosphorylated OmpR regulatory protein. false false _578_ 18858 18858 9 false None false Monica Prudencio annotation2530714 1 GFP range2530714 1 1 720 annotation2530719 1 RFP (codon optimized) range2530719 1 1003 1683 annotation2530718 1 BD21 range2530718 1 918 1002 annotation2530717 1 OmpR (R0082) range2530717 1 810 917 annotation2530716 1 BsaI sticky end range2530716 1 806 809 annotation2530715 1 BD24 range2530715 1 721 805 BBa_J100309_sequence 1 tcatcatttgtacagttcatccataccatgcgtgatgcccgctgcggttacgaactccagcagaaccatatgatcgcgtttctcgttcggatctttagacagaacgctttgcgtgctcagatagtgattgtctggcagcagaacaggaccatcaccgattggagtgttttgctggtagtgatcagccagctgcacgctgccatcctccacgttgtggcgaattttaaaattcgctttaatgccatttttttgtttatcggcggtgatgtaaacattgtggctgttaaaattgtattccagcttatggcccaggatattgccgtcttctttaaagtcaatgcctttcagctcaatgcggtttaccagggtatcgccttcaaatttcacttccgcacgcgttttgtacgtgccgtcatccttaaaggaaatcgtgcgttcctgcacatagccttccggcatggcggacttgaagaagtcatgctgcttcatatggtccggataacgagcaaagcactgaacaccataagtcagcgtcgttaccagagtcggccaaggtaccggcagtttaccagtagtacagatgaacttcagcgtcagtttaccattagttgcgtcaccttcaccctcgccacgcacggaaaacttatgaccgttgacatcaccatccagttccaccagaatagggacgacaccagtgaacagctcttcgcctttacgcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccccgactcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgagggatggtttctaatggcaagcagcgaagatgtgattaaagaatttatgcgcttcaaggtgcgtatggaaggtagcgttaatggtcatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatattccggattacctgaaattaagctttccggaaggttttaaatgggaacgcgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcttacaggatggtgagtttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgtctgaagctgaaagacggtggtcattatgatgcagaagttaaaaccacctacatggccaaaaaaccggttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagtaccggtgcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z