LOCUS       BBa_J100312             2338 bp    DNA     linear       01-Jan-2026
DEFINITION  repClone Red (J100205) with scrambled TetR promoter (J100311)
ACCESSION   BBa_J100312
VERSION     BBa_J100312.1  
FEATURES             Location/Qualifiers
     CDS             1..639
                     /label=TetR Repressor (C0041)
     RBS             640..727
                     /label=RBS BD24
     promoter        728..763
                     /label=P5 Promoter
     CDS             764..1483
                     /label=GFP
     RBS             1490..1501
                     /label=RBS B0034
     promoter        1516..1568
                     /label=Scrambled TetR promoter
     misc_feature    1519..1531
                     /label=5' TetR binding site
     misc_feature    1544..1555
                     /label=3' TetR binding site
     RBS             1573..1660
                     /label=RBS BD18
     CDS             1658..2338
                     /label=RFP
ORIGIN
        1 cggccgctac tagtattatt agctaccgct ttcacatttc agttgttttt ccagtccgca
       61 aataatcagt tctaaaccaa acagaaaggc aggttctgca ccctgatgat caaacagttc
      121 aatggcctga cgcagcagcg gaggcatgct atcggtggtc ggggtttcgc gttcttcttt
      181 tgcaacctga tgttcttgat cttccagaac acaacccagg gtaaaatgac caactgcgct
      241 cagtgcatac agtgcgtttt ccagactaaa accttgctga cacagaaatg ccagctgatt
      301 ttccagggtt tcatactgtt tttcggtcgg acgggtgccc agatgaactt ttgcaccatc
      361 acgatggctc agcagggcac aacgaaagct ttttgcatta ttacgcagaa aatcctgcca
      421 gctttcacct tccagcggac aaaaatgggt atgatgacga tccagcattt caattgccag
      481 tgcatccagc agtgcacgtt tgtttttaac atgccaatac agggtcggct gttcaacacc
      541 cagtttctgt gccagtttac gggtggtcag accttcaata ccaacttcat tcagcagttc
      601 cagtgcgcta ttaatcactt tgcttttatc cagacggctc attagaaacc gtccatcgca
      661 tgattaagat gtttcagtac gaaaattgct ttcattgttg atctcctttt taagtgaact
      721 tgggccctcc acataaatta cgagccggat gattaattgt caattattat ttgtatagtt
      781 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtgatctc
      841 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      901 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      961 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
     1021 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt
     1081 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
     1141 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
     1201 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
     1261 ggtatctcgc aaagcattga acaccataac cgaaagtagt gacaagtgtt ggccatggaa
     1321 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     1381 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
     1441 gaattgggac aactccagtg aaaagttctt ctcctttacg catctagtat ttctcctctt
     1501 taattactag acgactcctg ttatcacgaa gagattgaca tccagtgtta tacagagata
     1561 ctgagcacgc gggggcccaa gttcacttaa aaaggagatc aacaatgaaa gcaattttcg
     1621 tactgaaaca tcttaatcat gcgacggagc gtttctaatg gcttcctccg aagatgttat
     1681 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     1741 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     1801 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     1861 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     1921 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagat ggtggtgttg ttaccgttac
     1981 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     2041 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     2101 acgtatgtac ccggaagatg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     2161 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     2221 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagatta
     2281 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataa
//