BBa_J100312
1
BBa_J100312
repClone Red (J100205) with scrambled TetR promoter (J100311)
2016-12-02T12:00:00Z
2016-12-02T10:53:50Z
Assembled by inserting J100311 (scrambled TetR promoter) in repClone Red (J100205) using Golden Gate Assembly with BsaI.
We inserted a non-functional version of the TetR repressible promoter in repClone Red. Because the TetR binding sites have been scrambled, the promoter does not function to allow transcription of the downstream RFP, regardless of the presence of aTc. We will use this part as a negative control when using repClone Red to test the effects of modifications to the TetR promoter on its ability to promote transcription with or without aTc present.
false
false
_578_
18858
18858
9
false
N/A
false
Monica Prudencio
annotation2531670
1
RBS B0034
range2531670
1
1490
1501
annotation2531672
1
5' TetR binding site
range2531672
1
1519
1531
annotation2531673
1
3' TetR binding site
range2531673
1
1544
1555
annotation2531669
1
GFP
range2531669
1
764
1483
annotation2531675
1
RFP
range2531675
1
1658
2338
annotation2531671
1
Scrambled TetR promoter
range2531671
1
1516
1568
annotation2531667
1
RBS BD24
range2531667
1
640
727
annotation2531668
1
P5 Promoter
range2531668
1
728
763
annotation2531674
1
RBS BD18
range2531674
1
1573
1660
annotation2531666
1
TetR Repressor (C0041)
range2531666
1
1
639
BBa_J100312_sequence
1
cggccgctactagtattattagctaccgctttcacatttcagttgtttttccagtccgcaaataatcagttctaaaccaaacagaaaggcaggttctgcaccctgatgatcaaacagttcaatggcctgacgcagcagcggaggcatgctatcggtggtcggggtttcgcgttcttcttttgcaacctgatgttcttgatcttccagaacacaacccagggtaaaatgaccaactgcgctcagtgcatacagtgcgttttccagactaaaaccttgctgacacagaaatgccagctgattttccagggtttcatactgtttttcggtcggacgggtgcccagatgaacttttgcaccatcacgatggctcagcagggcacaacgaaagctttttgcattattacgcagaaaatcctgccagctttcaccttccagcggacaaaaatgggtatgatgacgatccagcatttcaattgccagtgcatccagcagtgcacgtttgtttttaacatgccaatacagggtcggctgttcaacacccagtttctgtgccagtttacgggtggtcagaccttcaataccaacttcattcagcagttccagtgcgctattaatcactttgcttttatccagacggctcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccctccacataaattacgagccggatgattaattgtcaattattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcatctagtatttctcctctttaattactagacgactcctgttatcacgaagagattgacatccagtgttatacagagatactgagcacgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z