BBa_J100313
1
BBa_J100313
Scrambled OmpR Partial Promoter (first 70 bp)
2017-01-19T12:00:00Z
2017-01-19T09:53:08Z
The original sequence is the OmpR promoter (R0082). We used an online scrambling tool to generate the new sequence (http://www.bioinformatics.org/sms2/shuffle_dna.html).
We scrambled the first 70 base pairs of the OmpR promoter (R0082). This should disrupt the transcription factor binding sites such that the promoter is no longer functional. We will insert this modified promoter in actClone (J100204) to serve as a negative control when testing the effects of modifications to the promoter.
false
false
_578_
18858
18858
9
Not in stock
false
N/A
false
Monica Prudencio
annotation2532804
1
Location of wt C2 OmpR
range2532804
1
34
51
annotation2532805
1
Location of wt C3 OmpR
range2532805
1
54
70
annotation2532803
1
Location of wt C1 OmpR
range2532803
1
13
30
BBa_J100313_sequence
1
tttttactactaataatactcatgatataatactagtacagcttagtttcgccagaatactagttagtat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z