BBa_J100314 1 BBa_J100314 actClone Red (J100204) with scrambled OmpR promoter (J100313) 2017-01-19T12:00:00Z 2017-01-19T09:57:19Z Assembled by inserting scrambled OmpR partial promoter (J100313) into actClone Red (J100204) using Golden Gate Assembly with BsaI. We inserted a non-functional version of the OmpR promoter in actClone Red. Because the OmpC binding sites have been scrambled, the promoter does not function to allow transcription of the downstream RFP. We will use this part as a negative control when using actClone Red to test the effects of modifications to the OmpR promoter on its ability to promote transcription. false false _578_ 18858 18858 9 Not in stock false N/A false Monica Prudencio annotation2532806 1 GFP range2532806 1 1 720 annotation2532811 1 RFP range2532811 1 1003 1683 annotation2532808 1 BsaI Sticky end range2532808 1 806 809 annotation2532807 1 BD24 range2532807 1 721 805 annotation2532809 1 OmpR Scrambled promoter range2532809 1 810 917 annotation2532810 1 BD21 range2532810 1 918 1002 BBa_J100314_sequence 1 tcatcatttgtacagttcatccataccatgcgtgatgcccgctgcggttacgaactccagcagaaccatatgatcgcgtttctcgttcggatctttagacagaacgctttgcgtgctcagatagtgattgtctggcagcagaacaggaccatcaccgattggagtgttttgctggtagtgatcagccagctgcacgctgccatcctccacgttgtggcgaattttaaaattcgctttaatgccatttttttgtttatcggcggtgatgtaaacattgtggctgttaaaattgtattccagcttatggcccaggatattgccgtcttctttaaagtcaatgcctttcagctcaatgcggtttaccagggtatcgccttcaaatttcacttccgcacgcgttttgtacgtgccgtcatccttaaaggaaatcgtgcgttcctgcacatagccttccggcatggcggacttgaagaagtcatgctgcttcatatggtccggataacgagcaaagcactgaacaccataagtcagcgtcgttaccagagtcggccaaggtaccggcagtttaccagtagtacagatgaacttcagcgtcagtttaccattagttgcgtcaccttcaccctcgccacgcacggaaaacttatgaccgttgacatcaccatccagttccaccagaatagggacgacaccagtgaacagctcttcgcctttacgcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccccgactttttactactaataatactcatgatataatactagtacagcttagtttcgccagaatactagttagtatcgtgttggattattctgcatttttggggagaatggactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgagggatggtttctaatggcaagcagcgaagatgtgattaaagaatttatgcgcttcaaggtgcgtatggaaggtagcgttaatggtcatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatattccggattacctgaaattaagctttccggaaggttttaaatgggaacgcgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcttacaggatggtgagtttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgtctgaagctgaaagacggtggtcattatgatgcagaagttaaaaccacctacatggccaaaaaaccggttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagtaccggtgcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z