LOCUS       BBa_J10050              1017 bp    DNA     linear       22-Dec-2024
DEFINITION  weak promoter + weak RBS + lacZa.GFP fusion
ACCESSION   BBa_J10050
VERSION     BBa_J10050.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     misc_feature    50..53
                     /label=conserved
     RBS             44..57
                     /label=BBa_B0031
     CDS             64..243
                     /label=lacZa
     misc_feature    244..300
                     /label=linker
     CDS             301..1017
                     /label=GFP
     misc_feature    64..1017
                     /label=BBa_E0051
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagtcacaca ggaaacctac
       61 tagatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      121 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      181 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      241 cgcgcgggcg gcagcgaagg cggcggcagc gaacatcatc atcatcatca tggcagcgaa
      301 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      361 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      421 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      481 actaccctga cctatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
      541 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      601 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      661 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      721 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
      781 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
      841 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      901 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      961 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataa
//