LOCUS       BBa_J10051              1015 bp    DNA     linear       22-Dec-2024
DEFINITION  weak promoter + strong RBS + lacZa.GFP fusion
ACCESSION   BBa_J10051
VERSION     BBa_J10051.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..241
                     /label=lacZa
     misc_feature    242..298
                     /label=linker
     CDS             299..1015
                     /label=GFP
     misc_feature    62..1015
                     /label=BBa_E0051
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      121 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      241 cgcgggcggc agcgaaggcg gcggcagcga acatcatcat catcatcatg gcagcgaacg
      301 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      361 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      421 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      481 taccctgacc tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
      541 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      601 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      661 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      721 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
      781 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
      841 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
      901 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
      961 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataa
//